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    Effect of energy deficiency at the end of pregnancy on sheep and lamb birth weights
    (Dicle Üniversitesi Veteriner Fakültesi, 2022) Durak, Mehmet Hanifi; Ateş, Atila; Bilal, Tanay
    This study was carried out in Chios breed pregnant sheep which created experimentally energy deficiency. To determine the effect of low energy feeding on the birth weights of sheep and lambs in the last period of pregnancy. 38 Chios breed sheep, 4-6 years old, were used in the study. The live weight weights of the ewes before the pregnancy were done on the zero day and the other weight weights on the days 120, 127, 134, 141 and 148 of the pregnancy. On the 105th day of pregnancy, pregnant and non-pregnant sheep were divided into 3 groups as normal energy pregnant group (PNE), deficient energy pregnant group (PLE) and non-pregnant normal energy group (N-PNE). From the 106th day of pregnancy to delivery; The pregnant sheep in the PNE group were fed with the ration containing 13% HP and 10 MJ/kg ME, the pregnant sheep in the PLE group were fed with ration containing 13% HP and 8.0 MJ/kg ME. The sheep in the N-PNE group were fed with ration containing 11% HP and 8.8 MJ/kg ME. PLE and N-PNE groups were found to have lower body weight than the PNE group. However, there was no statistical difference between these groups. In all pregnant sheep, body weight was higher than 120 days of pregnancy, it was found to be higher than day 0 (p <0.05) and after 120 days there was no statistically significant difference between groups. In the study, there was no statistically significant difference between the birth weights of PNE and PLE group. This can be explained by the fact that fetal growth is not affected by %13 HP ve 8.0 MJ/kg ME and does not cause serious energy deficiency in animals.
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    Genetic polymorphisms of Cyp19 and myostatin genes in Turkish indigenous sheep breeds
    (Hellenic Veterinary Medical Society, 2017) Akış, Iraz; Gürsel, Feraye Esen; Çakmak, Neziha Hacıhasanoğlu; Atmaca, Gizem; Yardibi, Hasret; Ateş, Atila; Durak, Mehmet Hanifi
    Growth and meat production traits are very important in sheep breeding. Cyp19 gene has a major role in reproductive activity and growth due to its function in estrogen synthesis. Another gene affecting growth traits is Myostatin (MSTN) gene, which mainly regulates skeletal muscle growth. In this study allele frequencies of genetic polymorphism in Cyp19 and Myostatin genes were identified by PCR-RFLP method in five indigenous Turkish sheep breeds, Chiose, Imroz, Kivircik, Zom and Morkaraman. Digestion of Cyp19 gene with HaeIII only revealed uncut AA genotype and digestion of MSTN with DraI also revealed only uncut AA genotype. Both loci analyzed in this study were found to be monomorphic in five Turkish indigenous sheep breeds. These highly conserved parts of the two genes can be useful for molecular evolutionary studies in sheep. Further studies regarding association analysis of Cyp19 and MSTN in sheep should be conducted.