Yazar "Alwasel, Saleh H." seçeneğine göre listele

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    Comprehensive metabolite profiling of berdav propolis using LC-MS/MS: Determination of antioxidant, anticholinergic, antiglaucoma, and antidiabetic effects
    (MDPI, 2023) Karageçili, Hasan; Yılmaz, Mustafa Abdullah; Ertürk, Adem; Kızıltaş, Hatice; Güven, Leyla; Alwasel, Saleh H.; Gülçin, İlhami
    Propolis is a complex natural compound that honeybees obtain from plants and contributes to hive safety. It is rich in phenolic and flavonoid compounds, which contain antioxidant, antimicrobial, and anticancer properties. In this study, the chemical composition and antioxidant activities of propolis were investigated; ABTS•+, DPPH• and DMPD•+ were prepared using radical scavenging antioxidant methods. The phenolic and flavonoid contents of propolis were 53 mg of gallic acid equivalent (GAE)/g and 170.164 mg of quercetin equivalent (QE)/g, respectively. The ferric ion (Fe3+) reduction, CUPRAC and FRAP reduction capacities were also studied. The antioxidant and reducing capacities of propolis were compared with those of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), α-tocopherol and Trolox reference standards. The half maximal inhibition concentration (IC50) values of propolis for ABTS•+, DPPH• and DMPD•+ scavenging activities were found to be 8.15, 20.55 and 86.64 μg/mL, respectively. Propolis extract demonstrated IC50 values of 3.7, 3.4 and 19.6 μg/mL against α-glycosidase, acetylcholinesterase (AChE) and carbonic anhydrase II (hCA II) enzyme, respectively. These enzymes’ inhibition was associated with diabetes, Alzheimer’s disease (AD) and glaucoma. The reducing power, antioxidant activity and enzyme inhibition capacity of propolis extract were comparable to those demonstrated by the standards. Twenty-eight phenolic compounds, including acacetin, caffeic acid, p-coumaric acid, naringenin, chrysin, quinic acid, quercetin, and ferulic acid, were determined by LC-MS/MS to be major organic compounds in propolis. The polyphenolic antioxidant-rich content of the ethanol extract of propolis appears to be a natural product that can be used in the treatment of diabetes, AD, glaucoma, epilepsy, and cancerous diseases.
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    Comprehensively revealing the profile of Pistacia vera L. cv. Siirt Turpentine - Antioxidant, Antidiabetic, Anti-Alzheimer, and Antiglaucoma effects
    (ACG Publications, 2023) Karageçili, Hasan; Yılmaz, Mustafa Abdullah; Alwasel, Saleh H.; Arık, Mustafa; Gülçin, İlhami
    Pistacia species containing turpentine which has protective effects and used as a medicine against diabetes, and infections for a long time. To interpret the antioxidant capacities of turpentine from Pistacia vera L. cv. Siirt, 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical (ABTS•+) and 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH?), Fe3+-2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) and Cu2+ reducing ability were studied and compared to reference antioxidants of BHT, BHA, ?-Tocopherol, and Trolox. The IC50 of turpentine for ABTS•+ and DPPH? scavenging capacities were determined to be lower than reference antioxidant standards as 231.1 ?g/mL and 346.6 ?g/mL, consequently. The total flavonoid and phenolic contents in turpentine were measured as 12.5 mg GAE/g and 38.852 mg QE/g, respectively. For determination of antidiabetic, anti-Alzheimer, and antiglaucoma effects of turpentine, acetylcholinesterase (AChE), ?-glycosidase and carbonic anhydrase II (hCA II) enzymes inhibition abilities of turpentine were performed. The IC50 values of turpentine extract against ?-glycosidase, hCA II, and AChE enzymes were found as 2.04, 3.59 and 14.6 µg/mL, respectively. The quantity of protocatechuic acid and naringenin were determined using LC-MS/MS against 53 standards. The results clearly demonstrated polyphenolics in P. vera L. cv. Siirt turpentine is a natural product has potential usage in amelioration of glaucoma, AD, diabetes, and epilepsy.