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    Comprehensive phenolic profiling and biological evaluation of Centaurea glastifolia L. (Asteraceae)
    (Taylor & Francis Ltd, 2024) Gozcu, Sefa; Aksit, Zeynep; Aydin, Ali; Yilmaz, Mustafa Abdullah; Simsek, Samed
    The present investigation focused on the comprehensive analysis of the phenolic profile of Centaurea glastifolia L. (Asteraceae) and the assessment of its diverse biological activities. Utilising LC-MS/MS, the phytochemical composition of the 70% methanol extract of Centaurea glastifolia (CG-ME) was thoroughly elucidated, revealing the presence of 30 distinct phytochemical compounds. Notably, major phenolic constituents identified in the extract included quinic acid, chlorogenic acid, luteolin-7-O-glucoside, kaempferol-3-O-glucoside, luteolin, and apigenin-7-O-glucoside. The antioxidant, antibacterial, antiproliferative, and cytotoxic activities of CG-ME were investigated. The CG-ME exhibited a moderate capacity for scavenging DPPH radicals (IC50: 50.05 +/- 1.58 mu g/mL) and FRAP (63.96 +/- 0.39 mg TE/g extract), indicating a moderate level of antioxidant activity. Moreover, CG-ME demonstrated significant antiproliferative effects (GI(50): 1.10 and 1.30 mu g/mL) on cancer cells (C6 and HTC cancer cell lines, respectively) while displaying low cytotoxicity towards normal cells (LC50: >1000 mu g/mL). In terms of antibacterial activity, CG-ME was found to be inactive against tested both Gram-positive and Gram-negative bacterial strains (MIC > 500 mu g/mL). The extracts had a promising antiproliferative effect on C6, HeLa, and HT29 cancer cell lines with a less cytotoxic effect (10.5-14.2%) against normal cells.
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    Exploring the Potential of Psephellus huber-Marathi (Wagenitz) Wagenitz: A Comprehensive UHPLC-MS/MS Analysis of Phytochemical Composition and Evaluation of Antioxidant, Antimicrobial, and Antiproliferative Activities
    (Kahramanmaras Sutcu Imam Univ Rektorlugu, 2024) Akman, Tugrul Cagri; Simsek, Samed; Aksit, Zeynep; Aydin, Ali; Yilmaz, Mustafa Abdullah
    Psephellus huber-morathii (PH) is an endemic species in the Eastern Anatolia Region. In this study, besides the biological activities of the 70% methanol extract of PH, its comprehensive phytochemical composition was investigated. The phenolic composition of the PH extract was analyzed using the UHPLC-MS/MS method. To evaluate its antimicrobial characteristics, the microdilution technique was employed. The antioxidant capabilities and total phenolic and flavonoid contents of the extract were determined using the spectrophotometer. Additionally, the effects of the extracts on cell proliferation and NCI-60 survival parameter values were assessed using the MTT assay. Quinic acid and chlorogenic acid were major compounds. The extract showed high antioxidant activity in DPPH (IC50:13.9 +/- 0.4 jig mL-1) and FRAP (61.3 +/- 2.3 mg TE g extract-1) methods. The total phenolic and flavonoid contents of the extract were found as 52.2 +/- 1.9 mg GAE g extract-1 and 28.6 +/- 0.9 mg QE g extract-1, respectively. Discussion: The extract showed low antiproliferative activity against normal lung and retinal cell lines, promising anticancer effects on gynecological and colon cancer cells, and moderate antimicrobial activity against Bacillus cereus and Enterococcus faecium. The study demonstrated the medicinal potential and value of PH extract against infectious diseases and cancer.