Yazar "Akkus M." seçeneğine göre listele

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    Intravesical oxybutynin application: Ultrastructural effects on bladder epithelium
    (1999) Ersay A.; Ketani M.A.; Nergiz Y.; Demirtas O.C.; Akkus M.; Otludil B.
    Intravesically applied oxybutynin, rapidly absorbs into the bloodstream, additional to profound local effect. Currently morphologic effects of oxybutynin on local bladder tissue relatively well established at light microscopic level, but not ultrastructural level. Thirty New Zealand White female rabbits were catheterized daily and intravesical instillation were performed with whether l mg/kg oxybutynin solution or saline for 30 days. The local effects of the drug on bladder epithelium at electron microscopic level were examined comparing with saline administration. Urinary tract infection (UTI) incidence were similar in both saline and oxybutynin groups (9 vs. 10 of 15 animals respectively) (p>0.05). Interestingly, in 4 of 5 animals that received oxybutynin and never had UTI during the study, separation of zonula occludens intercellular junction was determinated by Jeol electron microscope. This observation can explain rapid absorption of oxybutynin through the bladder into the blood stream. Oxybutynin can damage bladder surface epithelium at ultrastructural level. This effect may lead to increase absorption of it, but not result in higher incidence of bacterial infection.
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    Investigation of functional sperm membrane integrity after consequence of anti–hypoosmotic swelling test technique: An electron microscopic study
    (Journal of Reproductive Medicine, Inc., 2015) Tutsi Y.; Akkus M.; Tunik S.; Nergiz Y.; Kaplanoglu I.; Nasir Y.
    OBJECTIVE: Infertility is described as the inability of a couple to conceive after 1 year of unprotected intercourse. Male factor is assumed to be responsible for 50% of cases of infertile couples. It is a common clinical problem, affecting approximately 13– 15% of couples worldwide. In this study we aimed to investigate the effects of hypoosmotic swelling (HOS) and anti-HOS tests on sperm membrane integrity at the ultrastructural level. STUDY DESIGN: Twenty-nine infertile and 10 fertile men were included in this study. The fertile and infertile subjects were classified according to Kruger and WHO criterion. All semen samples were examined and analyzed based on WHO guidelines. Sperm viability was determined by using the eosin Y staining method. After HOS and anti-HOS tests were applied, the samples were evaluated at the ultrastructural level. RESULTS: Normal structural features of all regions of sperm were observed in sections of sham normospermia. Some histopathological changes were seen in HOS and anti-HOS group sections. CONCLUSION: The HOS procedure was found not to cause degenerative changes in the sperm ultrastructure. The anti-HOS procedure can be applied in normospermic and oligospermic groups. © Journal of Reproductive Medicine®, Inc.
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    Öğe
    Structural and immunophenotypic characterization of high endothelial venules in rat human tissue
    (2004) Akkus M.; Yuruker S.; Dagdeviren A.; Asan E.
    Objective: To present additional data on high endothelial venule (HEV) structure and immunophenotype. Methods: We used the zinc iodide-osmium tetroxide technique (ZIO), which is a metallophilic fixation and staining technique to examine HEVs at light and electron microscopic levels as this technique was previously reported to be reactive with cells in HEVs. Tonsils and lymph nodes were obtained from the Surgery and Otorhinolaryngology Departments, Hacettepe University Hospital, Ankara, Turkey during 2002 and 2003. An indirect immunohistochemical technique was used to examine frozen human tissue samples. Results: Organelle rich high endothelial cells, sheet-like processes of pericytes surrounding HEVs, structural relation of pericyte processes with fibroblastic reticular cells, an unusual multivesicular body-like organelle within high endothelial cells were presented. Expression of a large panel of defined and yet non-defined antigens on HEVs are also presented using an indirect immunoperoxidase technique. Conclusion: Presence of some of these antigens on HEVs was previously reported while no previous report is available for others. Significance of the expression of these antigens in HEVs, structural hints for trans endothelial migration of lymphocytes and their travel along the reticular cell meshwork is briefly discussed.