Yazar "Akdemir, Hulya" seçeneğine göre listele

Listeleniyor 1 - 6 / 6
  • [ X ]
    Öğe
    Cold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets
    (Springer Heidelberg, 2014) Koc, Ibrahim; Akdemir, Hulya; Onay, Ahmet; Ciftci, Yelda Ozden
    Genetic stability of plants during in vitro propagation and conservation is one of the important aspects of plant biotechnology. In the present study, micropropagated P. lentiscus L. shoot cultures, which are cultivated for the mastic resin, have been cold stored up to 12 months at 4 A degrees C in the dark for different durations (2, 4, 6, 8, 10 and 12 months) and genetic alterations in cold storage conditions were evaluated. Growth parameters such as proliferation rate, shoot numbers per explant, shoot lengths and shoot forming capacity were also calculated. Since the highest proliferation rate (100 %) was obtained in 6 month-stored shoot cultures without any severe influence of cold stress on proliferation ability, amplified fragment length polymorphism (AFLP) and inter-retrotransposon amplified polymorphism (IRAP) marker systems were used to determine genetic stability in the plantlets after this storage period. Totally, 702 scorable bands were produced by 10 AFLP primer pairs. Genetic similarity value of the non-stored (control) plant and cold-stored clones ranged from 0.66 to 0.84 with a mean of 0.74. In the case of IRAP, 159 bands were produced by 8 IRAP primers. Genetic similarity value of the non-stored plant and cold-stored clones varied from 0.65 to 0.83 and the average genetic similarity value was determined as 0.72. The genetic similarity indices revealed that genetic variability was similar in both techniques. Our results showed that tissue culture and especially cold storage of P. lentiscus L. may result transposons activation, thus could cause genetic instability.
  • [ X ]
    Öğe
    Detection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markers
    (Springer, 2016) Akdemir, Hulya; Suzerer, Veysel; Tilkat, Engin; Onay, Ahmet; Ciftci, Yelda Ozden
    Determination of genetic stability of in vitro-grown plantlets is needed for safe and large-scale production of mature trees. In this study, genetic variation of long-term micropropagated mature pistachio developed through direct shoot bud regeneration using apical buds (protocol A) and in vitro-derived leaves (protocol B) was assessed via DNA-based molecular markers. Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) were employed, and the obtained PIC values from RAPD (0.226), ISSR (0.220), and AFLP (0.241) showed that micropropagation of pistachio for different periods of time resulted in reasonable polymorphism among donor plant and its 18 clones. Mantel's test showed a consistence polymorphism level between marker systems based on similarity matrices. In conclusion, this is the first study on occurrence of genetic variability in long-term micropropagated mature pistachio plantlets. The obtained results clearly indicated that different marker approaches used in this study are reliable for assessing tissue culture-induced variations in long-term cultured pistachio plantlets.
  • [ X ]
    Öğe
    IN VITRO MICROGRAFTING OF THE ALMOND CULTIVARS TEXAS, FERRASTAR AND NONPAREIL
    (Taylor & Francis Ltd, 2013) Yildirim, Hakan; Akdemir, Hulya; Suzerer, Veysel; Ozden, Yelda; Onay, Ahmet
    A successful micrografting technique for the almond cultivars (cvs) Texas, Ferrastar and Nonpareil was developed using in vitro germinated seedlings as rootstocks and axenic shoot cultures established from mature tree sources as microscions. In vitro germinated seedlings, which developed 14 days after culturing in the modified Murashige and Skoog (MS) medium, were decapitated and used as rootstock. Shoot culture initiation from three almond cvs (Texas, Ferrastar and Nonpareil') was successfully achieved by culturing mature shoot tips from forced nodal buds, about 4-6 mm, on a modified MS medium containing I mg.L-1 benzyl adenin (BA). Slit micrografting on epicotyl and on hypocotyls were equally successful (83.3 % to 100 %). Grafting success was dependent on the rootstock type and lenght of the scion. Grafting success varied between 83.33 % and 100 % depending on the cultivar, when the scion contained I, 2, and 3 nodes. When almond scions, about 1.5 cm long, were micrografted on germinated seedling and cultured on proliferation medium (PM), the mean shoot length was 19.84 mm, 16.50 mm, 26.93 mm for the cvs Texas, Ferrastar and Nonpareil respectively Micro grafts could be easily cultured on a hormone-free semi-solid MS medium and were potted out after 4 to 6 weeks of culture growth. Rooted micrografted plantlets were successfully acclimatized and transferred to potting mix with 100 % survival. Although low percentages of variation were obtained in tested cvs (3.70 %, 6.25 % and 10.2 % in Texas, Ferrastar and Nonpareil'), molecular analysis showed that the developed micrografting technique produces genetically stable plantlets, at least up to 6 months of sub-culturing in cvs Ferrastar and Nonpareil. The described micrografting technique could be used for rejuvenation of shoot explants of mature elite almond cultivars and it also has potential use in the commercial production of other almond cultivars. Biotechnol. & Biotechnol. Eq. 2013, 27(1), 3493-3501
  • [ X ]
    Öğe
    Micropropagation of the pistachio and its rootstocks by temporary immersion system
    (Springer, 2014) Akdemir, Hulya; Suzerer, Veysel; Onay, Ahmet; Tilkat, Engin; Ersali, Yusuf; Ciftci, Yelda Ozden
    Although several studies have been reported on the micropropagation of the pistachio and its rootstocks, to date none of them had been efficient on the mass production of these plants in bioreactor systems. Thus, the micropropagation of juvenile pistachio shoot tips and nodal buds was investigated in a temporary immersion bioreactor system (RITA(A (R))) and on a conventional semi-solid medium. Among the tested immersion conditions, immersion for 24 min every 16 h reduced vitrification and improved proliferation in the pistachio. Interactions were evident in immersion time and frequency in nodal segments. Nodal buds were better than shoot tips as the highest multiple shoot formation was recorded in MS medium containing 4 mg L-1 BA and 0.1 mg L-1 GA(3) in RITA(A (R)). Although shoot tip necrosis (STN) was observed in shoots proliferated on semi-solid MS medium, such a symptom did not occur in shoots sprouted in the RITA(A (R)). Additionally, these optimized conditions were applied to nodal buds of mature male pistachio 'AtlA +/- aEuro (TM) and Pistacia rootstocks (P. khinjuk Stocks and P. atlantica Desf.), and the micropropagation in the bioreactor system, in comparison to the semi-solid medium, was also improved. Furthermore, in vitro rooting of pistachio plantlets, despite the lower range (27.5 %), was also achieved in RITA(A (R)). However, rooting was better on semi-solid medium for all tested species (ranged between 50 and 70 %). The results of this study showed that RITA(A (R)) could be used for the mass propagation of pistachio and its rootstocks, as well as for other woody plant species.
  • [ X ]
    Öğe
    Oxidative Stress of Mature Pistachio (Pistacia vera L. 'Atli') Shoot Tips During In Vitro Culture.
    (Springer, 2016) Akdemir, Hulya; Suzerer, Veysel; Tilkat, Engin; Onay, Ahmet; Ciftci, Yelda Ozden
    [Abstract Not Available]
  • [ X ]
    Öğe
    Plant tissue culture techniques-Tools in plant micro-propagation
    (Elsevier Sci Ltd, 2011) Onay, Ahmet; Yildirim, Hakan; Tokatli, Yelda Ozden; Akdemir, Hulya; Suzerer, Veysel
    [Abstract Not Available]