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Öğe The effect of phenolic amino acids on differentiation of DNA, RNA, proteins and cell growth in Cicer arietinum L.(Mbr Press Inc, 1999) Otludil, B; Akbayin, H; Taskin, T; Demir, RThe effect of three amino acids (phenilalanine tyrosine triptophane) have been examined on differentiation of DNA, RNA, protein synthesis and cell growth in cell culture for Cicer arietinum L. cultivated in Murashige and Skoog medium. The Cicer arietinum L, culture were treated with 20, 40,60,80 and 100 mu g. levels of each phenolic amino acid. Amount of DNA, RNA, proteins and cell growth were determined by the levels in the time intervals of 24 h incubation time during 6 days. Obtained data were evaluated statistically Consequently, experimental results reveal that excessive amount of phenolic amino acids cause significant inhibition on RNA, protein and cell growth. Phenilalanine not strongly effect on DNA synthesis or activity of DNA polymerase was unaffected by phenilalanine but tyrosine and triptophane has significant effect on DNA. On the other hand, it has been supposed that extreme amount of phenolic amino acids may be converted to phenolic compounds by cell regulatory systems.Öğe Effect of TSB supplemented with various concentration and combination of glucose and EDDA on slime production in Staphylococcus epidermidis(Mbr Press Inc, 1997) Elci, S; Atmaca, S; Gul, K; Akbayin, HSlime production by S.epidermidis was investigated in TSB and TSB supplemented with glucose, EDDA and combinations of glucose and EDDA in various concentrations. Fourteen clinically relavent strains were assayed and cultivated in these media at different incubation periods. The optical density scores obtained from ELISA autoreader were evaluated statistically. Most of S.epidermis strains were capable of switching to high level slime production when subjected to these media. Particularly, the low slime producer group of S.epidermis was stimulated to high level slime production by these media and increasing incubation period decreased slime production differences among the low, moderate and strong slime producer groups.Öğe The effects of magnesium, calcium and EDTA on slime production by Staphylococcus epidermidis strains(Springer, 2003) Akpolat, NÖ; Elçi, S; Atmaca, S; Akbayin, H; Gül, KEffect of magnesium, calcium and EDTA on slime production by 15 slime-positive and 13 slime-negative Staphylococcus epidermidis strains isolated from various clinical specimens was determined. The slime production on tryptic soy broth was significantly enhanced after addition of 128 mumol/L Mg2+. Similarly, the addition of Ca2+ caused a significant increase in slime production of all tested strains when concentration of Ca2+ exceeded 64 mumol/L. In contrast, in the presence of EDTA the slime production by all strains was significantly reduced. Hence Ca2+ and Mg2+ increase slime production of S. epidermidis. This finding is important in the context of the pathogenesis of biomedical implant infections caused by S. epidermidis.Öğe An experimental study on mycelial development period of some Pleurotus species, P-ostreatus, P-sapidus and P-sajor-caju, on the media that contain certain amount of barley crumb or wheat flour.(Mbr Press Inc, 1998) Akbayin, H; Yildiz, A; Ertekin, ASCultivation of Pleurotus species as mushroom is becoming important throughout the world because of their abilities to grow in a wide range of temperatures, on various types of medium and their shorter cultivation time. The purpose of this study is to determine whether the media that contain 1% malt extract and 1%, 1.5% or 2% wheat flour or barley crumb significantly decrease the mycelium development period of Pleurotus species, P. ostreatus, P. sapidus and P. sajor-caju, compared with the media that merely contains 1% malt extract and to establish which media is more appropriate to produce mycelia of these species vegetatively. As a result, of this study, the media that contain 1% barley crumb and 1% malt extract for P. ostreatus, 1.5% barley crumb and 1% malt extract for P. sapidus, and 1.5% barley crumb and 1% malt extract for P. sajor-caju has been suggested.Öğe Floristic note(Laser Pages Publ Ltd, 2000) Ertekin, AS; Akbayin, HTrifolium nigrescens Viv. subsp. petrisavii (Clem.) Holmboe var, grandifolium is identified as a new taxon collected from southwest Anatolia, Turkey.