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    In vitro micropropagation of almond (Amygdalus communis L. cv. Nonpareil)
    (Academic Journals, 2008) Isikalan, Cigdem; Akbas, Filiz Adiyaman; Namli, Suereyya; Tilkat, Engin; Basaran, Davut
    An efficient in vitro propagation method was developed for almond (Amygdalus communis L. cv. Nonpareil). The effect of BA and kinetin (0.0, 0.5, 1.0, 2.0, 4.0 mgl(-1)) on the culture initiation of zygotic embryos isolated from mature seeds was investigated. A Murashige and Skoog (1962) (MS) medium containing 30 gl(-1) sucrose, 0.5 and 1.0 mgl(-1) N-6-benzylaminopurine (BA) and 7 gl(-1) agar resulted in a multiple shoot initiation at the rate of 11.0 +/- 1.32 and 14.7 +/- 2.12 shoot per explant, respectively, in 28 days of culture. The effects of a low concentration of BA (0.1, 0.5, 1.0 and 2.0 mgl(-1)) and different combinations of auxin + cytokinin were investigated for shoot proliferation. The best results for new shoot production were obtained from a MS culture medium which was supplemented with 1.0 mgl(-1) BA. The rooting was achieved in a MS medium supplemented with 8.0 mgl(-1) indole acetic acid (IAA). The in vitro raised plants were acclimatized in a growth room and successfully transplanted to the field. This method here in described will be useful for the rapid multiplication of almond (A. communis L. cv. Nonpareil) in commercial exploitation.