Yazar "Akbalik M.E." seçeneğine göre listele

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    Immunohistochemical detection of estrogen and progesteron receptors in the bovine uterus and their relation to serum sex steroid hormone levels during the follicular and luteal phase
    (2009) Guney Saruhan B.; Sagsoz H.; Ketani M.A.; Akbalik M.E.; Ozyurtlu N.
    In this study, we tried to show the expression patterns of the steroid receptors in the bovine endometrium during the follicular and luteal phase. Samples of both uterus and blood were obtained from 30 adult, healty bovine at the moment of slaughter at a local slaughterhouse. Immunohistochemistry was performed by using rabbit polyclonal antibodies against the estrogen receptor (ER) and mouse monoclonal antibodies against the progesterone receptor (PR). In general, most of the uterine cells were stained positive but with a different intensity. During follicular phase, both ER and PR were obviously strong in the epithelia and the myometrium. For the glandular epithelium (GE), all GE cells were stained positive for ER and PR. When we compared both receptors during lutheal phase, a stronger intensity was observed in all compartments for PR, especially in the myometrium and surface-glandular epithelium. To summarize, the results from this study showed that both ER and PR might be regulated by the same mechanisms in some compartments and at specific stages of the oestrous cycle, and that each compartment of the uterus had a different expression of ER and PR which could accord with their different roles in reproductive physiology.
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    Öğe
    Immunohistochemical localisation of oestrogen and epidermal growth factor receptors of the bitch uterus in the sexual cycle
    (2010) Ozyurtlu N.; Sagsoz H.; Saruhan B.G.; Zonturlu A.K.; Ketani M.A.; Akbalik M.E.
    The localisation of oestrogen (ER) and epidermal growth factor receptors (EGFR) in the various cell types of the bitch uterine was determined. In this study, 23 adult, healthy crossbred bitches brought to the clinic for ovariohisterectomy were used. ER and EGFR positive staining was detected in all cell types of the uterus. A distinct staining was seen in the luminal and glandular epithelium; while stromal and myometrial cells showed weak or moderate staining. The endothelial and smooth muscle cells of the vessels in the endometrium and myometrium sometimes appeared positive. No staining was observed in the mesothelium. The results of this study suggested that ER and EGFR were expressed at various levels in different cell types of bitch uterus. In light of the previous studies, and data of the presented investigations, it may be necesssary to elicit the harmonious proliferation and differentiation of epithelial and stromal cells that are considered essential for the preparation of the uterus for implantation.