Yazar "Akalin Ş." seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • [ X ]
    Öğe
    The effects of alfa-tocopherol, ascorbic acid and pentoxifylline on bacterial translocation in common bile duct ligated rats
    (AVES, 2004) Aldemr M.; Geyik M.F.; Akalin Ş.; Hoşo?lu S.; Taçyildiz I.
    This study was conducted to investigate the effects of alfa-tocopherol, ascorbic acid and pentoxifylline on the bacterial translocation in an animal model of obstructive jaundice. Prolonged biliary obstruction gives rise to numerous changes in the liver and the host defense system. The increased biliary duct pressure may induce translocation of bacteria from the biliary tract into bloodstream and lymphatic system. Fifty rats were randomized into 5 groups containing 10 rats each. All rats were initially anaesthetized by intramuscularly injection of Ketamine (25 mg/kg). The abdomen was opened and the common bile duct was identified and divided after ligation using 5-0 silk. Sham operated rats (group 1) had a similar incision followed by mobilization of the common bile duct, without ligation or division. In group 2 rats, only common bile duct ligation was performed. In group 3, 4 and 5 rats common bile duct ligation was performed and alfa-tocopherol, ascorbic acid and pentoxifylline were administered for 21 days. The animals were sacrificed at the end of the 21st day, and serum levels of total bilirubin, ALT, AST, ALP and GGT were measured. Mesenteric lymph nodes, liver, spleen and blood were cultured. The bacterial translocations of rats in groups 1-5 were 0%, 82.5%, 25%, 30% and 20%, respectively. Bacterial translocation significantly increased in the mesenteric lymph nodes, liver, spleen and blood of rats in group 2 compared to group 1 (p<0.001). Bacterial translocations of the mesenteric lymph nodes, liver, spleen and blood in group 2 was significantly higher than those of group 3-5 (p<0.05). There was no significant difference between treatment groups (p>0.05). The predominant pathogens obtained from the mesenteric lymph nodes, liver, spleen and blood were Esherichia coli (42.9%), Klebsiella spp. (23.8%) and Proteus spp. (19%). The bacterial counts, colony-forming units per gram tissue (cfu/g), were found significantly higher in mesenteric lymph nodes, liver, spleen and blood of rats in group 2 than those of group 3, 4 and 5 (p=0.001). The present experimental study has demonstrated that, during obstructive jaundice, administration of alfa-tocopherol, ascorbic acid and pentoxifylline reduce the incidence of bacterial translocation.
  • [ X ]
    Öğe
    The effects of levofloxacin and intravenous immunoglobulins on sepsis due to Streptococcus pneumoniae in splenectomized rats
    (Turkish Surgical Society, 2003) Aldemir M.; Geyik M.F.; Akalin Ş.; Hoşo?lu S.; Taçyildiz I.; Ayaz C.
    This experimental study was conducted to investigate the effects of levofloxacin and intravenous immunoglobulins (IVIG) on sepsis due to Streptococcus pneumonia in splenectomized rats. Splenectomy impairs the immune response to bacterial infections. Postsplenectomy sepsis is a serious complication that can result in fulminant bacteriemia and even death. Sixty infant Sprague-Dawley rats were randomized into 5 groups containing 12 rats each. All rats were initially anaesthetized by intramuscularly injection of Ketamine (5 mg/kg). In the group 1, after the abdomen was opened, spleen was mobilized. In the other groups, splenectomy was performed. All of this animals (in the groups 2-5) were inoculated with Streptococcus pneumonia seven days after splenectomy. Fifteen hours after inoculation, blood (0.1 cc) was obtained for bacterial counts (cfu) and immediately drug therapy was given. The saline solution, levofloxacin, IVIG, levofloxacin plus IVIG were given in group 2-5 rats respectively. Twenty-four, 48 and 72 hours after initial therapy, blood samples were obtained from all rats for bacterial counts and biochemical examination (Ig G,A,M) and during 72nd hours rats were sacrificed. After inoculation in the groups 2-5 rats positive cultures were obtained. During 48th and 72nd hours, positive cultures (41.7% and 25%) of rats in groups 3 were significantly lower than (100% and 100%) those of group 2 (p<0.05 and p=0.001, respectively). Positive culture ratio (41.7%) in the group 4 was significantly lower than (100%) that of group 2 (p<0.05). During 24th, 48th and 72nd hours, positive cultures (41.7%, 16.7% and 0%) in the group 5 were significantly lower than those of group 2 (p<0.05, p<0.001 and p<0.001). During 24th, 48th and 72nd hours, bacterial counts in the groups 3-5 were significantly lower than those of group 2 (p<0.001). The IgG levels of groups 4 and 5 were significantly higher than those of groups 2 and 3 (p<0.001). The present experimental study has demonstrated that levofloxacin and IVIG, both alone and combine, are useful on sepsis due to S. pneumonia in splenectomized rats.