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    Effect of plant growth regulators on in vitro shoot multiplication of Amygdalus communis L. cv. Yaltsinki
    (Academic Journals, 2009) Akbas, Filiz; Isikalan, Cigdem; Namli, Suereyya; Ak, Bekir Erol
    An efficient protocol was established for in vitro shoot multiplication from apical shoot tips derived from mature trees of almond (Amygdalus communis L.) cultivars, Yaltsinki. Explants were cultured on Murashige and Skoog (1962) (MS) medium containing various concentrations of 6-benzyladenin (BA) and kinetin (kin) for shoot multiplication. Shoot multiplication was best achieved from explant on MS medium containing 30 gl(-1) sucrose, 7 gl(-1) agar and 1.0 mgl(-1) BA. This amount of BA (1.0 mgl(-1)) gave the best multiple shoot formation response with an average of 16.10 shoots per explant. In addition, shoots were cultured on the media containing 1.0 mgl(-1) BA and kin combined with three different auxins (0.25 and 0.5 mgl(-1) of IAA, IBA and NAA) separately. It was noted that 1.0 mgl(-1) BA and kin combinated with NAA had inhibitory effect on new shoot formation and no shoot formation was induced. However, explants cultivated on medium containing 1.0 mgl(-1) BA and 0.5 mgl(-1) IAA resulted in 11.25 shoots per explant. The effect of four different sucrose concentrations (20, 30, 40, 50 gl(-1)) on the multiplication of shoots was also investigated. The best shoot multiplication was obtained in MS media containing 30 gl(-1) sucrose with an average of 15.40 shoots per explant.
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    Öğe
    Micrografting of almond (Amygdalus communis) cultivar 'Nonpareil'
    (Southern Cross Publ, 2011) Isikalan, Cigdem; Namli, Sureyya; Akbas, Filiz; Ak, Bekir Erol
    Effects of plant growth regulators (PGRs) were investigated on micropropagation of scions and micrografting of almond (Amygdalus communis) cultivar 'Nonpareil'. In vitro germinated wild almond seedlings developed from seeds were used as rootstocks. The mature apical or subapical shoot tips of almond cultivar 'Nonpareil' were used as material for establishment of the microscions cultures. The shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.0, 0.5, 1.0, 1.5, 2.0, 4.0 mg l(-1)) of N-6-benzylaminopurine (BAP). The data showed that the increase in BAP concentration resulted in significant reduction at the shoot regeneration rate. Among all tested groups, the highest regeneration rate was obtained on medium containing 1.0 mg l(-1) BAP. The regenerated adventitious shoots from in vitro cultures were cultured on media containing BAP (0.5, 1.0, 1.5 mg l(-1)) combined with 0.2 and 0.4 mg l(-1) indole butyric acid (IBA) separately for development of shoots. The best respond was observed from MS medium supplemented with 1.0 mg l(-1) BAP + 0.2 mg l(-1) IBA. The effects of BAP and IBA (1.0 mg l(-1)) were studied on development of micrografted plantlets. Regenerated shoots tips, which were micrografted onto in vitro germinated wild almond seedlings. The results indicated that the most graft rate and new shoots formation were obtained 1.0 mg l(-1) BAP. In vitro micrografted plantlets were successfully transferred into commercial plastic pots for acclimatization.