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  • Küçük Resim
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    Effects of hypoxia on the mRNA expressions of TRAIL-mediated cell death related genes in hypoxia-tolerant rodent (Nannospalax nehringi) and some characteristics of these proteins
    (Inst Bioloska Istrazivanja Sinisa Stankovic, 2021) Yıldız, Barış; Kamiloğlu, Nadide Nabil; Öziç, Cem; Coşkun, Yüksel
    The aim of the present study was to determine the mRNA expression profiles of TRAIL-mediated cell death related genes of the hypoxia-tolerant rodent Nannospalax nehringi under hypoxia. The nucleotide and amino acid sequences of these genes were identified. Captured Nannospalax nehringi were randomly divided into normoxia and hypoxia groups. The hypoxia group was exposed to a 7% O-2 + 93% N-2 gas mixture for 52 h, while animals in the normoxia group were housed under normoxic conditions. Total RNAs were isolated from brain and lung tissues. The cIAP-1, cIAP-2, XIAP, DcR1, DcR2, FLIP and OPG genes were PCR amplified, and the cIAP-1, cIAP-2, XIAP, OPG, TRAIL and FLIP genes were sequenced. Sequenced genes were translated into amino acid sequences and compared with reliable sequences of closely similar species. The genes in the brain were regulated for protection against hypoxia; however, the genes in the lung were regulated differently. Many mutations and insertions were observed on the conserved regions of the cIAP-1, cIAP-2, XIAP, OPG and TRAIL genes in N. nehringi. We propose that these gene mutations and insertions contribute to the anti-hypoxic properties of N. nehringi
  • Küçük Resim
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    The effects of severe hypoxia on nitric oxide parameters ihypoxia-tolerant rodent: Nannospalax nehringi
    (Veteriner Fakultesi Dergis, 2019) Yıldız, Barış; Kamiloǧlu, Nadide Nabil; Öǧün, Metin; Öziç, Cem; Merhan, Oğuz; Mecit, Tarık; Coşkun, Yüksel
    Blind mole rats (BMRs) are solitary rodents which are tolerant to severe hypoxia. The aim of this study is to reveal the changes in nitric oxide (NO) and nitric oxide enzymes (NOS), which are involved in many physiological and pathological processes related with hypoxia, in BMRs under severe hypoxia. For this purpose, 12 subadult (11-15 moths) male Nannospalax nehringi were captured in Kars location. Captured BMRs were divided into two groups as Normoxic (NG) and Hypoxic (HG) randomly (n=6). NG were kept in completely dark, normoxic conditions for 52 h. HG were kept inside completely dark glovebox chamber with 7% oxygen flow for 52 h. After experimental protocol, NG were sacrificed under normoxic conditions and HG were sacrificed inside glovebox chamber with 7% oxygen. NO, iNOS, eNOS, nNOS and MDA levels of plasm and homogenized tissue samples were detected spectrophotometrically. All parameters of each sample were found to be high in HG compared to NG. But especially, NO was high in the lung tissues of HG. Additionally eNOS level of the kidney, liver and lung, iNOS levels of the liver and eNOS, and nNOS levels of the brain were found to be markedly high. Consequently, our data on NO and NOS enzyme production in Nannospalax nehringi tissues under hypoxia are compatible with the data obtained from other animals, but it contains differences in some points. We believe that these differences are different evolutionary adaptations of BMRs to hypoxia..