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    Effect of chronic low-intensity microwave radiation on sperm count, sperm morphology, and testicular and epididymal tissues of rats
    (Marcel Dekker Inc, 1999) Akdag, MZ; Çelik, MS; Ketani, A; Nergiz, Y; Deniz, M; Dasdag, S
    The aim of this study was to investigate the effect of chronic microwave exposure on sperm count, sperm morphology, and the morphology of rat testis and epididymes. Continuous wave microwave radiation (9450 MHz) specific absorption rate 1.80 W/kg and power density 2.65 mW/cm(2), was administered for 1 h a day for 13, 26, 39, and 52 days, which corresponded to 1, 2, 3, and 4 cycles of seminiferous epithelium, in mature male Sprague-Dawley rats. The rats were sacrificed under Ketalar anesthesia the day after exposure. Then epididymal sperm count, sperm morphology, and the weights of testis, epididymes, seminal vesicles, and prostate were determined. Histologic examinations of testis and epididymes were performed. The parameters were compared with sham groups. Epididymal sperm count decreased significantly only in the 52-day exposure group (p < 0.05). The percentage of abnormal sperm count changed significantly in the 26-, 39-, and 52-day exposure groups (p < 0.05, p < 0.05, and p < 0.001, respectively). The weights of testis and epididymes also changed significantly in the 26-, 39-, and 52-day exposure groups (p < 0.05, p < 0.05, and p < 0.05, respectively). Necrotic tubules, interstitial edema, perforated and necrotic tubules, decrease of spermatogenesis, and absent germinal epithelium in some tubules were observed in the exposed rat testes. In addition, we observed atrophy, interstitial edema, mononuclear cell infiltration, and increased fibroblastic activity in the exposed rat epididymes. We concluded that epididymal sperm count and morphology and weight and morphology of testis and epididymes were affected by chronic prolonged microwave exposure. The incidence of symptoms mentioned here depended on exposure duration.
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    Effects of whole body chronic 50 Hz sinusoidal weak magnetic field exposure on the rat pituitary hormones
    (Mbr Press Inc, 1999) Sert, C; Akdag, MZ; Çelik, MS; Dasdag, S
    We have investigated whether functions of pituitary gland change when the rats are exposed to sinusoidal magnetic fields at dark. In this study, 26 Wistar Albino rats were divided into two groups as experimental (n=14) and sham group (n=12). Experimental was exposed to 0.75 mT sinusoidal magnetic field procuded by Helmholtz coils and sham group was housed between inactivated Helmholtz coils, for one month. After one month Animals were sacrificed and their blood were taken. Serum hormone levels were determined by Radioimmunassay method. In exposed group, serum cortisol TSH, FSH and prolactin concentration did not statistically changed according to sham exposed group (p>0.05), but Growth Hormone and testesterone levels statistically changed (p<0.05).
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    Effects of whole-body chronic microwave exposure on some hormones of variously treated rats
    (Mbr Press Inc, 1999) Akdag, MZ; Sert, C; Kaya, H; Dasdag, S; Çelik, MS
    To investigate the effects of chronic whole-body microwave exposure, on serum Thyroid Stimulating Hormone (TSH), thyroxin (T-4), triiodothyronine (T-3), Cortisol, Growth Hormone (GH), Follicular Stimulating Hormone (FSH) and Estradiol concentrations, Wistar-albino female rats without treated and treated Vitamin C and Vitamin E were exposed to Microwave (MW) with a frequency of 9.450 MHz at the power density of 2.65 mW/cm(2) with corresponding specific absorption rate of 1.80 W/kg for 1h/day during 21 days. Vitamin C (150 mg/kg/day) and Vitamin E (150 mg/kg/day) were administrated during last seven exposure days to MW+Vit.C (n=8) and MW+Vit.E (n=8) groups respectively. Rectal temperatures were measured in all female rats. Serum levels of GH and FSH decreased (p<0.05, p<0.01 respectively) while estradiol levels increased in MW exposure group (p<0.05). But other hormones did not change in MW group (n=8). All hormone levels of rats treated with Vitamin C did not change (p>0.05). T-3 and T-4 hormones of exposed rats were found to be significant at MW +Vit.E group according to MW group (p<0.05). Rectal temperature differences were found to be significant in experimental groups according to sham group (n=8) (p<0.05). It was concluded that the changes of hormone concentrations under study was consistent with MW-induced thermal stimulation of Hypothalamic-Hypophysial-Adrenal (HHA) and Hypothalamic-Hypophysial-Thyroid (HHT) activity and Vitamin E might play role in changing T-3 and T-4 hormones.
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    Hormone levels of people occupationally exposed to radiofrequencies.
    (Mbr Press Inc, 1999) Dasdag, S; Balci, K; Kaya, H; Çelik, MS
    The present article intended to investigate some hormone levels of technicians in the radio-broadcasting, television (TV) and radio - link stations. The study was carried out on 43 technicians from 20 to 59 years age occupationally exposed to radiofrequency (RF) and microwave (MW) radiation. 20 unexposed and voluntary persons from 20 to 59 years age were accepted as a control group. We determined the levels of some hormones as triiodothyronine (T-3), tetraiodothyronine (T-4), thyroid stimulant hormone (TSH), free T-4, estradiol, dehydroepiondrosterone sulfate (DHEA), testosterone, cortisol and progesterone in the technicians under investigation. The hormone levels of exposure groups were statistically compared with the unexposed control group. T-3, T-4, estradiol, testosterone and progesterone levels of technicians in the radio-link and TV transmitter stations were found to be significant (p<0.05, p<0.001). T-3, T-4, TSH, estradiol and progesterone levels of technicians in the broadcasting station were also found to be significant (p<0.001). In conclusion some hormone levels of technicians under investigation were altered by RF/MW.