The effects of long-term exposure to extremely low-frequency magnetic fields on bone formation in ovariectomized rats

Abstract

The effects of long-term extremely low-frequency magnetic field (ELF-MF) exposure on bone formation and biochemical markers were investigated in ovariectomized rats. Sixty mature female SpragueDawley rats were randomly divided into four different groups (n?=?15): (i) unexposed control (CTL); (ii) ovariectomized only (OVX); (iii) non-ovariectomized, exposed (SHAM?+?ELF-MF); and (iv) ovariectomized, exposed (OVX?+?ELF-MF). The third and fourth groups were exposed to 1.5?mT ELF-MF for 4?h a day for 6 months. Bone mineral density (BMD) was determined using dual energy X-ray absorption (DEXA) measurements. The formation and resorption of bone were evaluated using bone-specific alkaline phosphatase (BAP), osteocalcin, osteoprotogerin, and N-telopeptide. After 6 months of ELF-MF therapy, BMD values were significantly lower in the OVX group and higher in the OVX?+?ELF-MF and SHAM?+?ELF-MF groups than they were before therapy (P?