Oluk E.A.Orhan S.Karakaş O.Çakir A.Gönüz A.2024-04-242024-04-2420101684-5315https://hdl.handle.net/11468/24637A method to induce indirect regeneration from Hypericum triquetrifolium Turra was described in the present study. Callus was induced from cotyledon explants of 35 days old aseptic seedlings on semi-solid MS supplemented with IAA (0.5 mg.L-1) combined with BAP (2 mg.L-1). Meristemoids developed on the surface of callus by decreasing of the cytokinins and plantlet regeneration with 100% frequency through these embryogenic calli occured in semi-solid medium when the PGRs were removed completely. Embryogenic calli obtained during the experiments were analyzed for their hypericin content. And they were found to produce hypericin as 48 ?g/g DM. Regenerated plantlets were rooted in MS containing 1 mg/L IAA. The highest percentages (94%) of survival of transferred plantlets to free-living circumstances were limited when they were acclimatized in sand: peat: perlite (1:1:1; v/v/v) mixture. © 2010 Academic Journals.eninfo:eu-repo/semantics/closedAccessHormone-Free MsHypericum TriquetrifoliumIndirect RegenerationArticle915222922332-s2.0-77951223288N/A