İnceören, NesrinAkay, FeryalNas, CemalDeveci, EnginKızıl, GökselKızıl, Murat2023-09-212023-09-212023İnceören, N., Akal, F., Nas, C., Deveci, E., Kızıl, G. ve Kızıl, M. (2023). Antiglycative effect of combination of extracts of capsicum annuum (chilli) and pyridoxamine against glycation in streptozotocin-induced experimental diabetes in rats. Revista Brasileira de Farmacognosia, 33(4), 831-846.1981-528Xhttps://link.springer.com/article/10.1007/s43450-023-00414-6https://hdl.handle.net/11468/12575Glycation causes the formation of advanced glycation end products, which play an important role in the pathogenesis of diabetic complications, which alter changes the structure and functional properties of proteins that negatively afect cellular metabolism. In the current study, we investigated benefcial efects of red chili pepper extract and capsaicin, the main bitter ingredient in red peppers and pyridoxamine, a vitamin B6, one by one or in combination on glycation and advanced glycation end products damage in streptozotocin-induced diabetic rats. The total amount of capcaicin in red chili pepper extract was found to be 3.51 mg/g DW by HPLC. Male Sprague Dawley rats (8 weeks old), weighing 200–300 g, were divided into 12 groups of 8 rats each. A single dose of streptozotocin (50 mg/kg body weight) was administered to induce experimental diabetes in rats. Group 1 was normal control, group 2 was diabetic control. Groups 3 and 4, 200 and 400 mg/kg red chili pepper extract, groups 5 and 6, 6 and 8 mg/kg capsaicin and group 7, 4 mg/ml pyridoxamine and group 8 were receiving 300 mg/kg metformin. Groups 9 and 10, 200 and 400 mg/kg red chili pepper extract+4 mg/ml PM and groups 11 and 12 were diabetic rats treated with 6 and 8 mg/kg capsaicin+4 mg/ml pyridoxamine. Oral treatment with intragastric tube was administered every day for 28 days. At the end of the experiment, subjects in all groups were given anesthesia once and the rib cage was performed by intracardiac intervention and blood was collected. Immediately, the liver and kidneys were grossly removed for histological examination. The results of our study showed that the combined doses of red chili pepper extract+pyridoxamine and capsaicin+pyridoxamine signifcantly reduced blood glucose levels, serum uric acid, urea, creatinine, AST, ALT, ALP, LDH, total cholesterol, fructosamine, HbA1c levels and signifcantly increased serum HDL and insulin levels. At the same time, histopathological studies also strongly supported the anti-diabetic, hepatoprotective and renoprotective efects of these combination doses.eninfo:eu-repo/semantics/closedAccessDiabetesAdvanced glycation end productsDiabetic nephropathyRed chili pepper extractPyridoxamineCapsaicinArticle334831846WOS:0010149597000022-s2.0-8516212257910.1007/s43450-023-00414-6Q2N/A