Dogru, MBaysal, ZAytekin, Ç2024-04-242024-04-2420060970-70770975-427Xhttps://hdl.handle.net/11468/20770The activity of proteolytic enzymes is commonly measured using casein as a substrate. Modified caseinolysis assay was developed with synthetic dyes such as procion red, procion yellow and procion blue for subtilisin carlsberg, protease type XVI, trypsin, chymotrypsin, respectively. Optimum pH, incubation time were determined. K-M, V-max and k(cat)/K-M values were also determined for these enzymes. The results indicate that the effect of all the tested enzymes on the synthetic dye-casein complexes; the most appropriate complex was found to be procion yellow.eninfo:eu-repo/semantics/closedAccessProteolytic ActivityDyed-CaseinSynthetic DyesSoluble SubstratesArticle18211741182WOS:0002366836000662-s2.0-33645546032Q4Q4