Kara, CigdemLacin, NihatAdak, Berat MetinKara, AlperTunik, SelcukUysal, ErsinSoylu, Fahri Emrah2025-02-222025-02-2220241019-5149https://doi.org/10.5137/1019-5149.JTN.43470-23.3https://hdl.handle.net/11468/29919AIM: To evaluate the effects of methylprednisolone and vitamin B12 injection on the regeneration of the nerves after a mental nerve crush injury. MATERIAL and METHODS: A total of 40 albino Wistar rats have been randomly divided into four groups: Ynt: ÖNEMLİ: Turkish Neurosurgery_JTN 43470_proof reading group 1 (n=10): crush-like nerve damage was created by pinching the left mental nerve for 60 s with an aneurysm clip and intraperitoneally administered 1 ml of saline for 14 days; group 2 (n=10): the left mental nerve was pinched for 60 s with an aneurysm clip and intraperitoneally administered 2 mg/kg of methylprednisolone for 14 days; group 3, experimental group (n=10): the left mental nerve was pinched for 60 s with an aneurysm clip and intraperitoneally administered 2 mg/kg of vitamin B12 for 14 days; and group 4, experimental group (n=10): the left mental nerve was pinched for 60 s and intraperitoneally administered 2 mg/kg of methylprednisolone and 2 mg/kg of vitamin B12 for 14 days. All rats were sacrificed on the 28th postoperative day, and histopathological evaluation was performed. RESULTS: Nerve damage was higher in the control group than in other groups (p<0.05). When Neural Cell Adhesion Molecule (NCAM) expression levels were compared, no major differences were observed between the methylprednisolone and control groups (p>0.05). The B12 and B12+methylprednisolone groups reached significantly higher NCAM expression levels compared to the control and methylprednisolone groups. When the myelin basic protein (MBP) expression levels were compared (p<0.05), the MBP expression was significantly higher in all experimental groups than in the control group (p<0.05). CONCLUSION: Systemic vitamin B12 and methylprednisolone administration effectively supported remyelination in the crushed mental nerve by increasing Schwann cell proliferation and differentiation. © (2024), (Turkish Neurosurgical Society). All rights reserved.eninfo:eu-repo/semantics/closedAccessMental nerve damageMethylprednisoloneNerve crush injuryNerve regenerationRatVitamin B12Article3457667722-s2.0-852043097073908728010.5137/1019-5149.JTN.43470-23.3Q3