Wranke, AnikaHeidrich, BenjaminErnst, StefanieSerrano, Beatriz CalleCaruntu, Florin AlexandruCurescu, Manuela GabrielaYalçın, Kendal2024-04-052024-04-052014Wranke, A., Heidrich, B., Ernst, S., Serrano, B. C., Caruntu, F. A., Curescu, M. G. ve diğerleri. (2014). Anti-HDV IgM as a marker of disease activity in hepatitis delta. Public Library of Science, 9(7), 1-13.1932-6203https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0101002https://hdl.handle.net/11468/13855Background: Hepatitis delta frequently leads to liver cirrhosis and hepatic decompensation. As treatment options are limited, there is a need for biomarkers to determine disease activity and to predict the risk of disease progression. We hypothesized that anti-HDV IgM could represent such a marker. Methods: Samples of 120 HDV-infected patients recruited in an international multicenter treatment trial (HIDIT-2) were studied. Anti-HDV IgM testing was performed using ETI-DELTA-IGMK-2-assay (DiaSorin). In addition, fifty cytokines, chemokines and angiogenetic factors were measured using multiplex technology (Bio-Plex System). A second independent cohort of 78 patients was studied for the development of liver-related clinical endpoints (decompensation, HCC, liver transplantation or death; median follow up of 3.0 years, range 0.6–12). Results: Anti-HDV IgM serum levels were negative in 18 (15%), low (OD,0.5) in 76 (63%), and high in 26 (22%) patients of the HIDIT-2 cohort. Anti-HDV IgM were significantly associated with histological inflammatory (p,0.01) and biochemical disease activity (ALT, AST p,0.01). HDV replication was independent from anti-HDV IgM, however, low HBV-DNA levels were observed in groups with higher anti-HDV IgM levels (p,0.01). While high IP-10 (CXCL10) levels were seen in greater groups of anti-HDV IgM levels, various other antiviral cytokines were negatively associated with anti-HDV IgM. Associations between anti-HDV IgM and ALT, AST, HBV-DNA were confirmed in the independent cohort. Clinical endpoints occurred in 26 anti-HDV IgM positive patients (39%) but in only one anti-HDV IgM negative individual (9%; p = 0.05). Conclusions: Serum anti-HDV IgM is a robust, easy-to-apply and relatively cheap marker to determine disease activity in hepatitis delta which has prognostic implications. High anti-HDV IgM levels may indicate an activated interferon system but exhausted antiviral immunity.eninfo:eu-repo/semantics/openAccessAnti-HDVHepatitis deltaArticle97113WOS:0003413076000052-s2.0-849049916422507284910.1371/journal.pone.0101002Q1Q1