Tural, B.Ertas, E.Enez, B.Tural, S.2024-04-242024-04-2420241735-14721735-2630https://doi.org/10.1007/s13762-024-05515-whttps://hdl.handle.net/11468/14981In this study, dead Bacillus niacini microorganisms were used to support the immobilization of magnetic iron nanoparticles, creating a magnetic nano-biosorbent for wastewater treatment through magnetic separation. Magnetic nano-biosorbent was characterized via scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectrophotometry, Brunauer-Emmett-Teller analysis, and vibration sample magnetometry techniques. The laser particle sizer confirmed a uniform distribution in the particle agglomerate sizes of magnetic iron nanoparticles and magnetic nano-biosorbent, affirming successful composite formation. Energy-dispersive X-ray spectroscopy confirmed Bacillus niacini specific elements, and Fourier transform infrared spectrophotometry indicated effective Bacillus niacini coating onto magnetic iron nanoparticles. Magnetic nano-biosorbent's efficacy for toluidine blue and lead (II) removal, considering pH, contact time, magnetic nano-biosorbent dosage, and initial pollutant concentrations, was assessed. Langmuir isotherms described toluidine blue and lead (II) biosorption optimally. Kinetic data matched the pseudo-first-order and pseudo-second-order models, implying multiple biosorption mechanisms. Magnetic nano-biosorbent displayed a biosorption capacity of 66.52 +/- 0.68 mg/g for lead (II) and 82.88 +/- 0.79 mg/g for toluidine blue. Reusability tests showed effective reuse for up to five cycles. The magnetic nano-biosorbent presents significant potential for wastewater treatment due to its high biosorption capacity, efficient removal, and cost-effective synthesis.eninfo:eu-repo/semantics/closedAccessBiomassMagnetic SeparationBiosorptionIsothermsKineticsReusabilityArticleWOS:0011786949000012-s2.0-8518690794710.1007/s13762-024-05515-wQ1N/A