Separation, optimization, and quantification of cytokinins by a recently developed amide-embedded stationary phase

Aral, HayriyeHasimi, DuyguAral, TarikLevent, AbdulkadirZiyadanogullari, Berrin2024-04-242024-04-2420171082-60761520-572Xhttps://doi.org/10.1080/10826076.2017.1333005https://hdl.handle.net/11468/16911In this study, some plant growth regulators known as cytokinins [kinetin (K), zeatin (Z), thidiazuron (TDZ), benzylaminopurine (BAP), and dimethylallylaminopurine (AAP)] were separated by HPLC using an amide-embedded mixed-mode stationary phase which was synthesized by Aral et al. in recent years. The effect of mobile phase content, mobile phase pH, buffer concentration, and temperature on separation process was studied. In addition, a quantitative determination of cytokinins from Salvia limbata extract was studied, and some validation parameters such as limit of detection (LOD), limit of quantification (LOQ), and relative standard deviation (RSD) were calculated as a range of 0.03-0.1, 0.1-0.26mg/L, and 0.03-0.08, respectively. [GRAPHICS] .eninfo:eu-repo/semantics/closedAccessAmide-Embedded Stationary PhaseCytokininsMixed-Mode Stationary PhasePlant Growth RegulatorsSalvia LimbataSeparation, optimization, and quantification of cytokinins by a recently developed amide-embedded stationary phaseSeparation, optimization, and quantification of cytokinins by a recently developed amide-embedded stationary phaseArticle4011549555WOS:0004091852000022-s2.0-8502194935210.1080/10826076.2017.1333005Q3Q4