Yavuz, MuratBaysal, Zubeyde2024-04-242024-04-2420130022-11471750-3841https://doi.org/10.1111/1750-3841.12018https://hdl.handle.net/11468/17470The objective of this study was to determine -casein adsorption by using supermacroporous poly(2-hydroxyethyl methacrylate-N-methacryloyl-(l)-histidine methyl ester) [p(HEMA-MAH)] cryogel. -Casein adsorption properties of p(HEMA-MAH) cryogel were studied for the application of -casein purification. The cryogel was produced by free radical polymerization initiated by N,N,N',N'-tetramethylene diamine and ammonium persulfate pairs in an ice bath. P(HEMA-MAH) cryogel was characterized by swelling tests, Fourier transform infrared spectroscopy, and scanning electron microscopy. The effects of the flow rate, pH, temperature, initial -casein concentration, and ionic strength on the adsorption efficiency of cryogel were studied. The equilibrium swelling degree of the p(HEMA-MAH) cryogel was 6.73g H2O/g cryogel. -Casein adsorption capacity of p(HEMA-MAH) cryogel from aqueous solution was estimated as 31.17mg/g cryogel. It was also observed that -casein could be repeatedly adsorbed and desorbed with p(HEMA-MAH) cryogel without significant loss in the adsorption capacity.eninfo:eu-repo/semantics/closedAccessAdsorption-CaseinChromatographyCryogelP(Hema-Mah)Article782E238E243WOS:0003149905000152-s2.0-848734206192333117610.1111/1750-3841.12018Q1Q2