Dogru, MBaysal, ZAytekin, ÇAydin, H2024-04-242024-04-2420061082-60681532-2297https://doi.org/10.1080/10826060500533984https://hdl.handle.net/11468/16905The activity of proteolytic enzymes is commonly measured using casein as a substrate. A modified caseinolysis assay was developed with natural dyes such as juglone, lawsone, berberine, and quercetin for Subtilisin carlsberg , protease type XVI, and trypsin, respectively. The pH dependence and incubation time were determined. K-m, V-max, and k(cat)/K-m values were also determined for these enzymes. Lawsone was found to be a better substrate than the others.eninfo:eu-repo/semantics/closedAccessProteolytic ActivityDyed-CaseinNatural DyesSoluble SubstratesArticle362165174WOS:0002356902000052-s2.0-336447926001651356010.1080/10826060500533984Q2Q4