Abdullah, Fuad O.Muhammad, Haval H.Yılmaz, Mustafa AbdullahÇakır, OğuzTarhan, Abbas2024-04-242024-04-242024Abdullah, F. O., Muhammad, H. H., Yılmaz, M. A., Çakır, O. ve Tarhan, A. (2024). LC-ESI-MS/MS analysis of phenolic compounds and in vitro cytotoxicity and apoptosis-inducing effects of Nonea pulmonarioides extracts. Natural Product Research, 1-8.1478-64191478-6427https://doi.org/10.1080/14786419.2024.2306914https://hdl.handle.net/11468/17001The chemical composition of Nonea pulmonarioides extracts were investigated for the first time. The phytoconstituents of the methanol extracts were screened by using LC/MS-MS technique. The anticancer activity of the acetone and methanol extracts were measured against four cancer cell lines; MCF-7, PC3, HT-29, and U-87 MG. Thirty phenolic compounds were identified, rosmarinic (90.06 mg analyte/g extract) and fumaric acids (39.737 mg analyte/g extract) were major compounds of the studied species. Moreover, both methanol and acetone extracts were found to have strong anticancer activities. The acetone extract HT-29 (with IC50 of 10.17 +/- 0.25 mu g/mL) compared with standard cis-platin (with IC50 of 22.20 +/- 0.72 mu g/mL) with apoptotic mediated programmed cell death. These findings identified N. pulmonarioides as a potential species exhibiting anticancer properties. In conclusion, the compelling results show that the methanol extract contains possible bioactive compounds with anticancer properties that require isolation and further characterisation. [GRAPHICS] .eninfo:eu-repo/semantics/openAccessMicrowave extractionAnalytical method validationLc-Ms/MsRosmarinic acidFumaric acidCytotoxicityApoptosisArticleWOS:0011500039000012-s2.0-851829976653826386410.1080/14786419.2024.2306914Q1N/A