Çiçek, Ayşegül ÇopurDüzgün, Azer ÖzadSaral, AyşegülKayman, TubaÇIzmecI, ZeynepBalcı, Pervin ÖzlemDal, Tuba2024-04-242024-04-242013Çiçek, A. Ç., Düzgün, A. Ö., Saral, A., Kayman, T., ÇIzmecI, Z., Balcı, P. Ö. ve diğerleri. (2013). Detection of class 1 integron in Acinetobacter baumannii isolates collected from nine hospitals in Turkey. Asian Pacific Journal of Tropical Biomedicine, 3(9), 743-747.2221-1691https://doi.org/10.1016/S2221-1691(13)60149-5https://hdl.handle.net/11468/23362https://www.sciencedirect.com/science/article/abs/pii/S2221169113601495Objective: To investigate the antibiotic resistance genes inserted into class 1 and class 2 integrons in Acinetobacter baumannii (A. baumannii) isolates obtained from nine different cities in Turkey. Methods: A collection of 281 A. baumannii clinical isolates were collected from nine diferent state hospitals in Turkey and were confirmed as A. baumannii by conventional biochemical, API testing and bla-OXA-51 specific PCR. The isolates were examined by PCR for existence of class 1 and 2 integron gene cassettes. Results: They were characterized by antimicrobial susceptibility testing and the highest resistance rates were determined for piperacillin (90.03%), ciprofloxacin (87.54%), cefepime and trimethoprim/sulfamethoxazole (81.13%). The lowest resistance rates was for cefotaxime (3.55%). class I integrons were detected in 6.4% (18/281) of A. baumannii strains and no class 2 integron was detected. The gene cassettes of class 1 integrons AacC1-AAC(3) I-aadA1, AacC1-aadA1, AAC(3)-I, AAC(3)-I -AAC(3)-I -aadA1, TEM-1, AAC(3)-I-aadA1 - AAC(3)-I -AAC(3)-I, AAC(3)-I -AAC(3)-I -AAC(3)-I -aadA1, AAC(3)-I - aadA1, AAC(3)-I-AAC(3)-I, AAC(3)-I-aadA1-AAC(3)-I-aadA1, AAC(3)-I-AAC(3)-I-aadA1-AAC(3)-I-aadA1 were detected in eighteen strains. The aac genes family were most frequently found integrated into the class 1 integrons and it was followed by aadA genes and TEM-1 genes. Conclusions: This is an extensive study on the distribution of class 1 integron among A. baumannii in Turkey. In addition to these, two new alleles were observed. Their percentage rates of similarity to other cassettes are 95% aadA1 (TKA18) and 89% aadA1 (ANKA3).eninfo:eu-repo/semantics/openAccessAcinetobacter baumanniiClass 1 integronGene cassetteResistanceArticle397437472-s2.0-848832572012399801710.1016/S2221-1691(13)60149-5Q2