Ünlü, İlkerTutal Gürsoy, GörkemDeveci, Engin2025-02-222025-02-2220240326-2383https://hdl.handle.net/11468/29939Spinal cord injury (SCI) is developed by primarily traumatic external causes or secondarily hemorrhage and edema in cord. SCI causes neurological and multiorgan dysfunction. In the present study, effects of honokiol was investigated on cerebellar tissue in experimental SCI model by biochemical and histochemical analysis. Thirty-male Sprague Dawley rats were assigned to three groups. SCI was developed by dropping a steel rod with a diameter of 3 mm and a weight of 10 g was dropped from 10 cm to the spinal cord T7-9 vertebras under anesthesia. Upon SCI, 50 mg/kg honokiol was intraperitoneally introduced to animals for postoperative 7 days. Cerebellar tissue samples were fixed in zinc-formalin and processed for further histochemical analysis. Blood samples were collected for malondialdehyde (MDA), total antioxidant status (TAS) and oxidant status (TOS). Results were statistically analyzed and recorded. MDA and TOS values were higher and TAS values were lower in SCI compared to sham group. Honokiol restored the values close to values of sham group. Histopathological scores were increased in SCI group but honokiol treatment alleviated the pathologies and restored scores in SCI+honokiol group. IL-6 and Bax expression was mainly negative in sham groups. Glial cells, Purkinje cells and granular cells of cerebellum showed negative IL-6 and Bax immune reaction. SCI increased the expression of IL-6 and Bax in basket cells, Purkinje cells, glia cells of the substantia alba, in ganglions layer. After SCI, honokiol administration reduced the immune reaction of IL-6 and Bax in cerebellar cells and substansia alba. SCI caused hypoxia and tissue degeneration and inflammation in cerebellum. Honokiol administration may prevent cell degeneration and apoptosis by balancing cytokine activity to prevent oxidative stress and may be an alternative in cerebellar therapy. © 2024, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.eninfo:eu-repo/semantics/closedAccesscerebellumhonokiolinflammationspinal cord injuryArticle4335715772-s2.0-85191022192Q4