Güler, EmineAkbulut, HüseyinBozokalfa, GulizDemir, BilalEyrilmez, Gizem OymanYavuz, MuratDemirkol, Dilek Odacı2024-04-242024-04-242015Güler, E., Akbulut, H., Bozokalfa, G., Demir, B., Eyrilmez, G. O., Yavuz, M. ve diğerleri. (2015). Bioapplications of polythiophene-g-polyphenylalanine-covered surfaces. Macromolecular Chemistry and Physics, 216(18), 1868-1878.1022-13521521-3935https://doi.org/10.1002/macp.201500219https://hdl.handle.net/11468/14089https://onlinelibrary.wiley.com/doi/10.1002/macp.201500219The fabrication of electro and bioactive surfaces by electrochemical deposition of the thiophene-functionalized polyphenylalanine macromonomer (T-g-PPhe) is reported. The resulting conducting graft copolymer, polythiophene-graft-polyphenylalanine (PT-g-PPhe) formed on the indium tin oxide (ITO) glass surface, is characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and fluorescence microscopy. Then, possible uses of PT-g-PPhe as matrices in the sensor design for both electrochemical biosensing and cell adhesion studies are investigated. In the first part, PT-g-PPhe that is deposited on ITO is further functionalized with the arginylglycylaspartic acid peptide via 1-Ethyl-3-(3 dimethylaminopropyl) carbodiimide for the selective cell adhesion. Immunofluorescence staining is performed to detect the difference between adherences of integrin alpha v beta 3 receptor positive (U87-MG) and negative (HaCaT) cell lines on to the biofunctional surface. In the second part, an electrochemical glucose sensor is constructed by immobilizing glucose oxidase on the surface of PT-g-PPhe, which is deposited on a glassy carbon electrode.eninfo:eu-repo/semantics/closedAccessCell adhesionElectropolymerizationGraft copolymerPolypeptide macromonomerSurface designArticle2161818681878WOS:0003625570000042-s2.0-8494181024010.1002/macp.201500219Q2Q2