Ucar, Eyyup H.Cetin, HayrettinAtli, Mehmet O.2024-04-242024-04-2420180093-691X1879-3231https://doi.org/10.1016/j.theriogenology.2018.01.018https://hdl.handle.net/11468/16127This study investigated molecular regulation in the canine corpus luteums/corpora lutea (CL) following multiple low-dose prostaglandin F-2 alpha (PGF(2)alpha) injections in non-pregnant bitches around 30-35 days after ovulation. The CL were obtained by ovariohysterectomy 1 h after the last PGF(2)alpha injection. The subjects were divided into the following groups: control (no PGF(2)alpha injection, n = 4), one PGF(2)alpha injection (injection at 0 h, 1PGF, n = 4), two PGF(2)alpha injection (injection at 0 and 8 h, 2PGF, n = 4), and three PGF(2)alpha injection (injection at 0, 8 and 24 h, 3PGF, n = 4). In the 1PGF group, the steady-state mRNA levels of an immediate early gene (NR4A1) and immune system-related genes (MCP-1 and IL-8) increased. NR4A1 was localized in luteal and endothelial cells. In contrast, MCP-1 was localized in the luteal tissue between the luteal and endothelial cells. LHCGR, CYP11A1, and StAR mRNA expression decreased after the second PGF(2)alpha injection. FASLG increased only after the third PGF(2)alpha injection. The mRNA levels of PTGFR, PGT, and PTGS2 decreased as the number of PGF(2)alpha injections increased. Immunohistochemistry showed a decrease in StAR protein density as the number of PGF(2)alpha injections increased. BAX and CASP3 mRNA expression levels were similar among the groups. Serum progesterone (P-4) levels decreased dramatically after the PGF(2)alpha injections but were still higher than the basal level at the end of the study. In conclusion, repeated low-dose PGF(2)alpha injections could induce luteolytic mechanisms in the CL of non-pregnant bitches. Furthermore, it can be concluded that, in non-pregnant bitches, some aspects of the molecular regulation of luteolysis in the CL are similar to some aspects of such regulation in other domestic animals. (C) 2018 Elsevier Inc. All rights reserved.eninfo:eu-repo/semantics/closedAccessProstaglandinf2 AlphaLutelysisGene ExpressionsCorpus LuteumBitchesArticle1133443WOS:0004310919000052-s2.0-850420542392945429610.1016/j.theriogenology.2018.01.018Q1Q1