Isikalan, CigdemAkbas, Filiz AdiyamanNamli, SuereyyaTilkat, EnginBasaran, Davut2024-04-242024-04-2420081684-5315https://hdl.handle.net/11468/21000An efficient in vitro propagation method was developed for almond (Amygdalus communis L. cv. Nonpareil). The effect of BA and kinetin (0.0, 0.5, 1.0, 2.0, 4.0 mgl(-1)) on the culture initiation of zygotic embryos isolated from mature seeds was investigated. A Murashige and Skoog (1962) (MS) medium containing 30 gl(-1) sucrose, 0.5 and 1.0 mgl(-1) N-6-benzylaminopurine (BA) and 7 gl(-1) agar resulted in a multiple shoot initiation at the rate of 11.0 +/- 1.32 and 14.7 +/- 2.12 shoot per explant, respectively, in 28 days of culture. The effects of a low concentration of BA (0.1, 0.5, 1.0 and 2.0 mgl(-1)) and different combinations of auxin + cytokinin were investigated for shoot proliferation. The best results for new shoot production were obtained from a MS culture medium which was supplemented with 1.0 mgl(-1) BA. The rooting was achieved in a MS medium supplemented with 8.0 mgl(-1) indole acetic acid (IAA). The in vitro raised plants were acclimatized in a growth room and successfully transplanted to the field. This method here in described will be useful for the rapid multiplication of almond (A. communis L. cv. Nonpareil) in commercial exploitation.eninfo:eu-repo/semantics/closedAccessAmygdalus Communis L. Cv. NonpareilMicropropagationZygotic EmbryosIn VitroArticle71218751880WOS:0002573587000132-s2.0-46349102412N/AQ4