Wickramasinghe, JanakaKaya, Can AyhanBeitz, DonaldAppuhamy, Ranga2024-04-242024-04-2420222076-2615https://doi.org/10.3390/ani12121474https://hdl.handle.net/11468/19265Simple Summary We demonstrated previously that supplementation of glutamine (Gln) at 2.0% of dry matter intake (DMI) increased the rate at which dairy calves achieved >= 1.0 kg/d starter feed intake (SFI) during weaning. Because Gln supplements at <1.0% of DMI or branched-chain amino acid (BCAA) supplements have been shown to improve the performance of weaning piglets, we examined the effects of a lower dose of Gln (8.0 g/d equivalent to 1% of DMI) alone or in combination with BCAA supplementations on SFI and average daily gain (ADG) in this study. Amino acids did not affect SFI or ADG during the supplementations but decreased post-weaning SFI in an additive manner even though the ADG was not affected. The blood analysis on the last day of supplementations revealed a possibility for the Gln and BCAA supplementations to suppress SFI through leptin and serotonin secreted by the gastrointestinal tract. The study objective was to examine the effects of supplementing Gln and BCAA on the SFI and ADG of weaning dairy calves. Holstein heifer calves (11 calves /treatment) at 35 d of age were assigned to: (1) no amino acids (CTL), (2) Gln (8.0 g/d) alone (GLN), or (3) Gln (8.0 g/d) and BCAA (GLNB; 17.0, 10.0, and 11.0 g/d leucine, isoleucine, and valine, respectively) supplementations in whole milk during a stepdown weaning scheme. Calves were weaned completely once they achieved >= 1.0 kg/d SFI. Neither GLN nor GLNB affected SFI or ADG in the first week during weaning. The GLNB decreased SFI compared to CTL, but the SFI was similar between CTL and GLN in the remainder of the weaning scheme. All calves were weaned at 50 d of age. The SFI of GLNB was lower than that of GLN, and the SFI of both GLN and GLNB were lower than CTL post-weaning. The decreased SFI did not alter ADG during weaning or post-weaning. The GLNB tended to have higher plasma leptin and lower plasma serotonin concentrations compared to CTL. Glutamine and BCAA seem to affect the SFI of calves by modulating the secretions of endocrine cells in the gastrointestinal tract.eninfo:eu-repo/semantics/openAccessBranched-Chain Amino AcidsDairy CalvesGlutamineStarter Feed IntakeArticle1212WOS:0008175914000012-s2.0-851313047883573981010.3390/ani12121474Q1Q1