Uçmak, FeyzullahEkin, Nazımİbiloğlu, İbrahimArslan, SerkanKaplan, İbrahimŞenateş, Ebubekir2024-04-042024-04-042016Uçmak, F., Ekin, N., İbiloğlu, İ., Arslan, S., Kaplan, İ. ve Şenateş, E. (2016). Prophylactic administration of silybin ameliorates L-arginine-induced acute pancreatitis. Medical Science Monitor, 22, 3641-3646.1234-1010https://medscimonit.com/abstract/index/idArt/898014https://hdl.handle.net/11468/13825Background: Oxidative stress have been shown to play a role in the pathogenesis of acute pancreatitis. The aim of this study was to investigate the potential effect of silybin, a potent antioxidant, on L-arginine-induced acute pancreatitis in an experimental rat model. Material/Methods: Forty female Wistar Albino rats were divided into 5 groups as follows: Group 1 (C): control group (n=8), Group 2 (SL): silybin group (n=8), Group 3 (LA): acute pancreatitis group (n=8), Group 4 (SLLA): prophylaxis group (n=8), and Group 5 (LASL): treatment group (n=8). Group C (control) received 2 intraperitoneal (i.p.) injections of physiological saline at an interval of 1 h. Group SL received only a single i.p. injection of silybin. The SLLA group received a single i.p. injection of silybin before the induction of acute pancreatitis with L-arginine, whereas the LASL group received the same injection after the induction of acute pancreatitis with L-arginine. Pancreatic tissues were histopathologically examined. Levels of amylase and oxidative stress markers (total oxidant status and total anti-oxidant status) were determined in the blood samples. Oxidative stress index was calculated. Results: In comparison to the LA, the prophylaxis and treatment groups showed significant improvements in serum oxidative stress parameters (p=0.001 and p=0.005, respectively). Histopathological analysis showed that the treatment group had significant improvements in edema scores only (p=0.006), whereas the prophylaxis group had the same improvements in inflammation and necrosis scores as well as in total scores (p=0.004, 0.006, and 0.004, respectively). Conclusions: When used for prophylactic rather than therapeutic purposes, silybin ameliorates serum oxidative stress parameters and improves histopathological results via its antioxidant and anti-inflammatory properties.eninfo:eu-repo/semantics/openAccessAcute necrotizingAntioxidantsArgininePancreatitisSilymarinArticle2236413646WOS:0003851345000012-s2.0-849914907882772562710.12659/MSM.898014Q1Q3