Yıldız, GülsümKaratoprak, Gökçe Şekerİlgün, SelenYılmaz, Mustafa AbdullahKöse, Yavuz BülentZengin, Gökhan2024-03-262024-03-262023Yıldız, G., Karatoprak, G. Ş., İlgün, S., Yılmaz, M. A., Köse, Y. B. ve Zengin, G. (2023). The relationship between the chemical components and skin-related enzyme inhibition, antioxidant activities and toxicity profile of Helichrysum armenium subsp. armenium. Plant Biosystems, 158(1), 119-129.1126-3504https://www.tandfonline.com/doi/full/10.1080/11263504.2023.2287587https://hdl.handle.net/11468/13727The aerial parts of Helichrysum armenium subsp. armenium were subjected to methanol, ethyl acetate and n-hexane extraction. The extracts were evaluated for their in vitro enzyme inhibitory activity [lipoxygenase (LOX), tyrosinase and elastase], antioxidant activity and toxicity (on fibroblast cells). All the extracts displayed a weak effect on LOX and elastase. The ethyl acetate extract showed the highest inhibition on tyrosinase enzyme with IC50 = 460 µg/mL, while kojic acid had IC50 = 30 µg/mL. The methanol and ethyl acetate extracts compared to the n-hexane extract exhibited stronger DPPH• and ABTS•+ scavenging activity, as well as iron(II)-chelating potential, and were shown to have rich phenolic and flavonoid contents. The methanol extract was toxic to healthy fibroblast cells at a higher concentration with IC50 > 500 µg/mL compared to the other extracts. Phytochemical analysis of the extracts was made quantitatively by LC–MS/MS. The major components identified in the methanol and the ethyl acetate extracts were astragalin, quinic acid, apigenin-7-glycoside, isoquercitrin, chlorogenic acid, naringenin, apigenin and luteolin-7-glucoside. The compounds detected in the n-hexane extract were much fewer. Based on these findings, H. armenium subsp. armenium can be considered to be a natural raw material with the potential to be used in the cosmetic industry.eninfo:eu-repo/semantics/closedAccessAntioxidantEnzyme inhibitoryHelichrysum armenium subsp. armeniumLC–MS/MSToxicityArticle1581119129WOS:0011246545000012-s2.0-8517999892510.1080/11263504.2023.2287587Q1N/A