Ortakaya, VeysiFincan, Sema Agüloğlu2024-04-242024-04-2420191308-53011308-8084https://doi.org/10.5505/biodicon.2019.70288https://search.trdizin.gov.tr/yayin/detay/318400https://hdl.handle.net/11468/28101In our study, the isolation, identification and optimization of Bacillus subtilis from soil samples were performedand the ability to produce ?-amylase of industrial importance was investigated. Bacteria identified as B. subtilis by 16SrRNA analysis, morphological and biochemical tests. Maximum growth was determined at 32 hour, 37°C and pH 7.0.Enzyme optimization was performed after determination of bacterial amylase in starchy medium. Optimum conditionsfor amylase production were determined as 37°C, pH 7.0 and 48 h. When the effect of metals on enzyme activity wasexamined, it was determined that Cu2+ increased enzyme activity, Zn2+, Hg2+ and Fe2+ were partially inhibited theenzyme activity, and Ca2+ was showed close activity to the control. It was observed that carbon sources had inhibitoryeffect on bacterial growth and enzyme production.eninfo:eu-repo/semantics/openAccessArticle123576431840010.5505/biodicon.2019.70288