Ergüç, AliOkur, HayatiKarakuş, FuatAlbayrak, GökayArzuk, EgeBaykan, Şüra2025-02-222025-02-2220232458-9179https://doi.org/10.30569/adiyamansaglik.1325975https://search.trdizin.gov.tr/tr/yayin/detay/1231556https://hdl.handle.net/11468/30122Aim: This study aims to evaluate the alterations in Oxypeucedanin (OXY)-mediated anticancer activity in different media. Second aim is to predict the affinity of OXY to electron transfer chain (ETC) complexes. Materials and Methods: MTT and LDH leakage assays were performed with OXY. Molecular docking studies were also conducted to predict the affinity of OXY to ETC complexes. Results: 250 µM OXY reduced viability in glucose media. ≥50 µM OXY decreased viability in galactose media. ≥50 µM OXY increased membrane disruption in galactose media. Molecular docking studies also showed that OXY might possess the capacity to bind to the inhibition sites of Complex I and IV. Conclusion: Galactose-conditioned media exacerbated the OXY-mediated cytotoxicity. Preliminary results suggested that mitotoxicity might take part in anticancer activity. Furthermore, OXY might cause ETC dysfunctions due to selective inhibition of Complex I and IV.eninfo:eu-repo/semantics/openAccessAnticancer activityIn silicoOxypeucedaninMitotoxicityArticle93153161123155610.30569/adiyamansaglik.1325975