Söğütçü, NilgünYokuş, Beran2022-08-172022-08-172021Söğütçü, N. ve Yokuş, B. (2021). Effects of gallic acid on ischemia-reperfusion induced testicular injury in a rat testicular torsion model. Analytical and Quantitave Cytopathology and Histopathology, 43(1), 1-7.0884-6812https://hdl.handle.net/11468/10184WOS:000640456900001OBJECTIVE: Testicular torsion is a common urological complication mostly affecting adolescents and young men. In this study we investigated biochemical and immunohistochemical effects of gallic acid on the damage induced by testicular torsion-detorsion. STUDY DESIGN: Forty male rats were divided into 4 groups of 10 animals each: control, torsion, torsion/detorsion, and torsion/detorsion + gallic acid. Testicles were removed from the scrotum, and 2.5-hour ischemia was applied to the left testis by twisting the spermatic cord 720 degrees clockwise. Then 3-hour reperfusion was allowed for detorsion. Gallic acid 20 mg/kg was orally administered to the torsion/detorsion + gallic acid group before reperfusion. Biochemical parameters of testicular tissue (MDA, SOD, CAT, and GSH levels) were measured. Testicular tissues were immune-stained with caspase-3 and TNF-alpha antibody. RESULTS: MDA levels in the torsion/detorsion + gallic acid group were close to those in the control group; however, it was higher in the torsion/detorsion group as compared to the control group. Compared to control group, SOD, CAT, and GSH activities were significantly increased in the torsion/detorsion + gallic acid group. However, those values were decreased in the torsion-detorsion group. Spermatogenic cells and interstitial cells showed positive caspase-3 expression in the torsion and torsion-detorsion groups; however, expression level was decreased in the torsion/detorsion + gallic acid group. TNF-alpha expression was observed in degenerated spermatogonia, Leydig cells, and macrophages in the torsion and torsion-detorsion groups. In the torsion/detorsion + gallic acid group, TNF-alpha expression was observed in some interstitial region rather than in cells of seminiferous tubules. CONCLUSION: Gallic acid treatment could be an alternative therapy in testicular ischemia-reperfusion injury to decrease inflammation, germ cell degeneration, and apoptosis.eninfo:eu-repo/semantics/closedAccessDetorsionIschemia-reperfusion injuryOxidative stressRatsWistarReperfusion injurySpermatic cord torsionSpermatogenesisTesticular torsionTestisTorsion abnormalityTorsion-detorsionArticle43117WoSIDEksikScopusIdYokN/AN/A