Poli, AnnaritaGuven, KemalRomano, IdaPirinccioglu, HamsiGuven, Reyhan GulEuzeby, Jean Paul MarieMatpan, Fatma2024-04-242024-04-2420120022-12601349-8037https://doi.org/10.2323/jgam.58.437https://hdl.handle.net/11468/18902A new thermophilic spore-forming strain Ge1(T) was isolated from the Guclukonak hot spring in Sirnak, Turkey. The strain was identified by using a polyphasic taxonomic approach. Strain Ge1(T) was Gram-positive, spore-forming, alkaliphilic rod-shaped, motile, occurring in pairs or filamentous. Growth was observed between 30 and 65 degrees C (optimum 60 degrees C) and at pH 5.5-10.0 (optimum pH 9.0). It was capable of utilizing starch, growth was observed at 0-3% NaCl (w/v) and was positive for catalase and urease. The major cellular fatty acids were iso-C-15:0 and iso-C-17:0, and the predominant lipoquinone found was menaquinone MK7 type. The DNA G+C content of the genomic DNA of strain Ge1(T) was 52.0%. Comparative 16S rRNA gene sequence studies showed that the isolate belonged to the genus Geobacillus. The DNA-DNA hybridization mean values between the representative strain Ge1(T) and the closely related species G. subterraneus, G. thermodenitrificans, G. thermocatenulatus, G. vulcani and G. thermoleovorans were 69.3%, 57%, 37%, 27% and 26%, respectively. The results of DNA-DNA hybridization, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Ge1(T). Based on these results, we propose assigning a novel subspecies of Geobacillus subterraneus, to be named as Geobacillus subterraneus subsp. aromaticivorans subsp. nov. with the type strain Ge1(T) (DSM 23066 (T)= CIP 110341(T)).eninfo:eu-repo/semantics/openAccessDna-Dna Hybridization AnalysisGeobacillus Subterraneus Subsp Aromaticivorans Subsp Nov.LipidsTaxonomyThermophileArticle586437446WOS:0003137559000052-s2.0-848738313842333757910.2323/jgam.58.437Q3Q4