Karatas, HakanUyar, FikretTolan, VeyselBaysal, Zubeyde2024-04-242024-04-2420131590-42611869-2044https://doi.org/10.1007/s13213-012-0443-6https://hdl.handle.net/11468/14938Eight different agro-residues were tested for alpha-amylase and protease production by using Bacillus licheniformis ZB-05. Among them, rice husk (RH) was proved as the best substrate for two enzymes (alpha-amylase 443 U/g and protease 469,000 U/g). Maximum enzyme production was observed to be 30 % initial moisture, with a growth period of 36 h in 20 and 30 % inoculum volumes for alpha-amylase and protease, respectively. The best enzyme recovery from solid mass was obtained when extracted with tap water. Among the tested various nitrogen sources, 1 % ammonium sulphate followed by 2 % Bacto liver, 2 % ammonium sulphate and 1 % Bacto casaminoacid served as the best inorganic and organic nitrogen sources for alpha-amylase and protease production, respectively. As additional carbon sources, 2 % soluble starch enhanced alpha-amylase production, while 1 % maltose enhanced protease production.eninfo:eu-repo/semantics/openAccessAlpha-AmylaseAgricultural WasteBacillus LicheniformisEnzyme ProductionProteaseSolid-State FermentationArticle6314552WOS:0003146924000042-s2.0-8487371862710.1007/s13213-012-0443-6Q2Q4