Gozcu, SefaAksit, ZeynepAydin, AliYilmaz, Mustafa AbdullahSimsek, Samed2025-02-222025-02-2220241478-64191478-6427https://doi.org/10.1080/14786419.2024.2403028https://hdl.handle.net/11468/29714The present investigation focused on the comprehensive analysis of the phenolic profile of Centaurea glastifolia L. (Asteraceae) and the assessment of its diverse biological activities. Utilising LC-MS/MS, the phytochemical composition of the 70% methanol extract of Centaurea glastifolia (CG-ME) was thoroughly elucidated, revealing the presence of 30 distinct phytochemical compounds. Notably, major phenolic constituents identified in the extract included quinic acid, chlorogenic acid, luteolin-7-O-glucoside, kaempferol-3-O-glucoside, luteolin, and apigenin-7-O-glucoside. The antioxidant, antibacterial, antiproliferative, and cytotoxic activities of CG-ME were investigated. The CG-ME exhibited a moderate capacity for scavenging DPPH radicals (IC50: 50.05 +/- 1.58 mu g/mL) and FRAP (63.96 +/- 0.39 mg TE/g extract), indicating a moderate level of antioxidant activity. Moreover, CG-ME demonstrated significant antiproliferative effects (GI(50): 1.10 and 1.30 mu g/mL) on cancer cells (C6 and HTC cancer cell lines, respectively) while displaying low cytotoxicity towards normal cells (LC50: >1000 mu g/mL). In terms of antibacterial activity, CG-ME was found to be inactive against tested both Gram-positive and Gram-negative bacterial strains (MIC > 500 mu g/mL). The extracts had a promising antiproliferative effect on C6, HeLa, and HT29 cancer cell lines with a less cytotoxic effect (10.5-14.2%) against normal cells.eninfo:eu-repo/semantics/openAccessCentaurea glastifolia LantiproliferativeantioxidantantibacterialLC-MS/MSArticleWOS:0013119230000012-s2.0-852041236853926735710.1080/14786419.2024.2403028Q1Q3