Tolan, VEnsari, Y2024-04-242024-04-2420060301-12080975-0959https://hdl.handle.net/11468/20746Endosulfan, a chlorinated hydrocarbon insecticide of cyclodiene subgroup acts as a contact poison in a wide variety of organisms. In the present study, the effect of endosulfan on the growth, alpha amylase activity and plasmid amplification was investigated in Bacillus subtilis system. The bacteria were grown in medium, incubated with different concentrations (32, 48, 64 and 80 mu g/mL) of endosulfan. The bacterial growth was gradually seen after 1st day at up to 48 mu g/l. endosulfan. The 48 mu g/L endosulfan inhibited approximately 50% of the bacterial growth. No growth was observed at and after 64 mu g/L endosulfan, for all days (1-5). Also, no a amylase activity was found in the supernatant of the Culture medium containing 64 and 80 mu g/L endosulfan, whereas slight activity was observed with 32 and 48 mu g/L endosulfan concentration. The amount of plasmid increased up to 50% in the presence of 32 mu g/L endosulfan. Endosulfan had no effect oil the alpha amylase activity in-vitro.eninfo:eu-repo/semantics/closedAccessEndosulfanBacillus SubtilisAlpha AmylasePlasmidArticle432123126WOS:0002372336000082-s2.0-3374916480016955761Q3Q4