Öncü, ŞeymaKızılkaya, Merve BecitBilir, AbdulkadirSarıtaş, AlperenSöylemez, Evrim Suna ArıkanKoca, Halit BuğraFırat, FatmaKaplan, Afife Büşra UğurYılmaz, Mustafa Abdullah2024-10-172024-10-172024Öncü, Ş., Kızılkaya, M. B., Bilir, A., Sarıtaş, A., Söylemez, E. S. A., Koca, H. B. ve diğerleri. (2024). Anti-Cataract effect of the traditional aqueouse extract of yerba mate (Ilex paraguariensis A. St.-Hil.): An in ovo perspective. Life, 14(8), 1-15.2075-1729https://www.mdpi.com/2075-1729/14/8/994https://hdl.handle.net/11468/28855Introduction: The therapeutic effect of different doses of the traditional aqueous extract of dried leaves of yerba mate (Ilex paraguariensis A. St.-Hil.) was investigated in an experimental cataract model in chicken embryos. Methods and Results: LC-MS/MS analysis allowed the identification and quantification of 53 metabolites. In the hydrocortisone-induced cataract model, lenses were examined morphologically after treatment and parameters related to oxidative stress (total antioxidant/oxidant status (TAS/TOS), glutathione (GSH), and malondialdehyde (MDA)) were evaluated. Antiproliferative cell nuclear antigen (PCNA) and caspase-3 H-scores were determined and crystallin alpha A (CRYAA) gene expression in the lenses was measured by RT-PCR. The degree of cataract decreased in all treatment groups. While there was no significant difference in TAS levels compared to the negative control, TOS, GSH, and MDA levels were dose-dependently regulated. Treatment groups other than the high-dose group regulated the decrease in PCNA and the increase in caspase-3. CRYAA gene expression increased significantly only at the lowest dose. Conclusion: YM, which is becoming increasingly popular as a traditional tea, showed a therapeutic effect on hydrocortisone-induced cataracts in chicken embryos at relatively low doses.eninfo:eu-repo/semantics/openAccessCataractChick embryoCRYAAIlex paraguariensisYerba mateArticle148115WOS:0013072809000012-s2.0-8520260056639202736Q1Q1