Karageçili, HasanPolat, TuğbaYılmaz, Mustafa AbdullahFidan, MehmetKaraismailoğlu, Mehmet CengizGülçin, İIhami2025-02-222025-02-222024Karageçili, H., Polat, T., Yılmaz, M. A., Fidan, M., Karaismailoğlu, M. C. ve Gülçin, İ. (2024). Evaluation of the antioxidant, antidiabetic and anti-alzheimer effects of Capsella bursa-pastoris-Polyphenolic Profiling by LC-MS/MS. Records of Natural Products, 18(6), 643-662.1307-6167https://doi.org/10.25135/rnp.489.2410.3353https://hdl.handle.net/11468/29594Capsella bursa-pastoris species of the Capsella herb were examined in this study for sreening of antioxidant, antidiabetic and anti-Alzheimer effects. Traditionally, people consumed C. bursa-pastoris against famine and satisfy their hunger. C. bursa-pastoris species have been shown to have utility as food, medicine, and industrial materials after extensive investigation. The antioxidant properties of methanol and water extracts of C. bursa-pastoris species were assessed using; Fe3+-2,4,6-tris(2-pyridyl)-S-triazine (TPTZ), ferric (Fe3+), and cupric (Cu2+) iobs reducing assays, as well as 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radical (ABTS(center dot+)) and N,N-dimethyl-p-phenylenediamine (DMPD) radical scavenging activities. The antioxidant and reducing capacity of C. bursa-pastoris species aerial parts water and methanol extracts were compared with standard antioxidants. The phenolic and flavonoid contents in methanol and water extracts of C. bursa-pastoris species were measured 6.86 to 12.00 mg GAE/g and 61.67 to 145.0 mg QE/g, respectively. The inhibitions of effects of water and methanol extract of C. bursa-pastoris species against alpha-amylase, and acetylcholinesterase (AChE) enzymes were investigated. The IC50 values were found as 168.6 to 238.6 mu g/mL against alpha-amylase and 19.0 to 20.9 mu g /mL against AChE. The number of fenolik compounds in both extracts of C. bursa-pastoris species were recorded using LC-MS/MS. The major phenolic and flavonoid components detected in methanol extract of C. bursa-pastoris were quinic acid (22.629 mg/g), chlorogenic acid (3.211 mg/g), rutin (1.930 mg/g), hesperidin (0.893 mg/g), and isoquercitrin (0.783 mg/g). In a similar order, quinic acid (11.356 mg/g), cyranoside (9.463 mg/g), chlorogenic acid (6.072 mg/g), hesperidin (5.912 mg/g), and isoquercitrin (5.364 mg/g) were found as plentyfull pehenolic antioxidants in methanolic extract. The results clearly demonstrated that polyphenolic antioxidants-rich ingredients of the aerial parts of C. bursa-pastoris species are biological phenolic comounds have persuasive usage in the medication of diabetes and Alzheimer's diseases.eninfo:eu-repo/semantics/openAccessCapsella bursa-pastorisEnzyme inhibitionAntioxidant activityAlpha-amylaseAcetylcholinesterasePhenolic compoundsArticle186643662WOS:0013856873000062-s2.0-8521423954910.25135/rnp.489.2410.3353Q2Q3