EFFICIENT MASS PROPAGATION OF ORIGANUM ACUTIDENS (HAND-MAZZ.) IETSWAART UNDER IN VITRO CONDITIONS
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Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Bangladesh Botanical Soc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study describes germination of Origanum acutidens (Hand.-Mazz.) letswaart seeds followed by micropropagation from stem nodes of 58 days old seedlings that were cultured on MS basal medium containing 0.4, 0.8, 1.2, 1.6, and 2.0 mg/I BAP with or without 0.5 mg/I NAA cultured at 24 degrees C 1 degrees C under cool white fluorescent lights with 16 hrs light (42 Mol photons/m(2)/s) photoperiod. The results showed maximum number of 10.28 shoots per explant on MS medium containing 0.8 mg/I BAP. Comparing regeneration on MS medium containing various concentrations of BAP with and without 0.5 mg/1 NAA; it was noted that shoot regeneration on MS medium containing BAP + NAA was partially inhibitory and induced comparatively reduced number of shoots per explant. Well developed shoots of 0. acutidens on each concentration of BAP with or without NAA were rooted on MS medium supplemented with 0.75 mg/I NAA. The rooted plantlets-were acclimatised in pots, followed by transfer to fields, where they bloomed and set seeds. The results suggest efficient practical production of the plant species for commercial purposes.
Açıklama
Anahtar Kelimeler
Origanum Acutidens, Plant Growth Regulators, Micropropagation
Kaynak
Bangladesh Journal of Botany
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
46
Sayı
2
