Effect of proteolytic treatment on dyeing of casein with synthetic dyes
[ X ]
Tarih
2006
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Asian Journal Of Chemistry
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The activity of proteolytic enzymes is commonly measured using casein as a substrate. Modified caseinolysis assay was developed with synthetic dyes such as procion red, procion yellow and procion blue for subtilisin carlsberg, protease type XVI, trypsin, chymotrypsin, respectively. Optimum pH, incubation time were determined. K-M, V-max and k(cat)/K-M values were also determined for these enzymes. The results indicate that the effect of all the tested enzymes on the synthetic dye-casein complexes; the most appropriate complex was found to be procion yellow.
Açıklama
Anahtar Kelimeler
Proteolytic Activity, Dyed-Casein, Synthetic Dyes, Soluble Substrates
Kaynak
Asian Journal of Chemistry
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
18
Sayı
2
