Is there a role for antioxidants in prevention of pulmonary hypoplasia in nitrofen-induced rat model of congenital diaphragmatic hernia?

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dc.contributor.authorCigdem, Murat Kemal
dc.contributor.authorKizil, Goksel
dc.contributor.authorOnen, Abdurrahman
dc.contributor.authorKizil, Murat
dc.contributor.authorNergiz, Yusuf
dc.contributor.authorCelik, Yusuf
dc.date.accessioned2024-04-24T16:00:12Z
dc.date.available2024-04-24T16:00:12Z
dc.date.issued2010
dc.departmentDicle Üniversitesien_US
dc.description.abstractMany studies suggest a role for antioxidants in the prevention of lung hypoplasia in nitrofen-induced rat models with congenital diaphragmatic hernia (CDH). This study investigates the oxidative status and the histological outcome of prenatal administration of vitamins E and C with synergistic effect, and effect of N-acetylcysteine (NAC) to improve lung maturation of nitrofen-induced rats. CDH was induced by maternal administration of a single oral dose of nitrofen on day 9.5 of gestation, and the Sprague-Dawley rats were randomly divided into five groups: nitrofen (N), nitrofen + vitamin C (NC), nitrofen + vitamin E (NE), nitrofen + vitamin C + vitamin E (NCE) and nitrofen + NAC (NNAC). A control group in which only vehicle was administered was included. Cesarean section was performed on day 21. Body weight (BW) and total lung weight (LW) of all fetuses with CDH were recorded; lung histological evaluation was performed, and protein content of lungs, determination of thiobarbituric acid reactive substances, and the protein carbonyls in tissue samples were determined. A total of 133 rat fetuses with CDH were investigated. The body weight and the lung weight of fetuses of all groups that were exposed to nitrofen were significantly decreased than of the control group (P < 0.05). The animals exposed to nitrofen with different antioxidants showed increased protein levels in lung tissue. However, in the NCE and the NNAC groups, protein levels were significantly increased than in the others. Malondialdehyde levels significantly decreased in the NCE and the NNAC groups when compared with the NC and the NE groups. In addition, the NCE and NNAC groups decreased protein oxidation to control levels, and no significant difference was observed between control and these two antioxidants groups. The N, NC, NE and NNAC groups showed minimal improvement in lung histology; the NCE groups showed the most improvement in lung histology when compared with the other nitrofen plus antioxidant groups. Prenatal administration of NAC and vitamin E in combination with vitamin C represented the best effects to avoid oxidative damage and protein content of the lungs in rat pups with CDH at birth.en_US
dc.identifier.doi10.1007/s00383-010-2552-2
dc.identifier.endpage406en_US
dc.identifier.issn0179-0358
dc.identifier.issn1437-9813
dc.identifier.issue4en_US
dc.identifier.pmid20127338
dc.identifier.scopus2-s2.0-77953928905
dc.identifier.scopusqualityQ2
dc.identifier.startpage401en_US
dc.identifier.urihttps://doi.org/10.1007/s00383-010-2552-2
dc.identifier.urihttps://hdl.handle.net/11468/14406
dc.identifier.volume26en_US
dc.identifier.wosWOS:000275749700010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPediatric Surgery International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNitrofenen_US
dc.subjectCdhen_US
dc.subjectPulmonary Hypoplasiaen_US
dc.subjectAntioxidant Vitaminsen_US
dc.subjectN-Acetylcysteineen_US
dc.subjectRaten_US
dc.subjectProtein Oxidationen_US
dc.subjectLipid Peroxidationen_US
dc.titleIs there a role for antioxidants in prevention of pulmonary hypoplasia in nitrofen-induced rat model of congenital diaphragmatic hernia?en_US
dc.titleIs there a role for antioxidants in prevention of pulmonary hypoplasia in nitrofen-induced rat model of congenital diaphragmatic hernia?
dc.typeArticleen_US

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