Phytochemical analysis and biological evaluation of hypericum linarioides bosse: in vitro and in silico studies
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this study is to determine the phenolic composition of Hypericum linarioides Bosse, to assess its various biological properties, and finally to confirm the experimental data of enzyme inhibitions via docking studies. LC-MS/MS analysis performed over methanol extract (ME) revealed the presence of 27 phytochemicals. Antioxidant activity results indicated that ME had the strongest DPPH radical scavenging and ferric ion reducing capacity than water and hexane extracts. XTT assays revealed the strongest anti-proliferative activity of ME against MCF-7 and HT-29 cancer cells. Flow cytometry results also confirmed the notable apoptotic effect of ME on MCF-7 cells through mitochondria-dependent pathway. Enzyme activity results exhibited the most promising acetylcholinesterase and alpha-glycosidase inhibitory activities of the ME. Molecular docking calculations of alpha-glycosidase with the major compounds (1, 15, 17 and 19) indicated that 17 (isoquercitrin) had the best docking binding energy (-10.92 kcal/mol) with appropriate binding interactions.
Açıklama
Anahtar Kelimeler
Biological activity, Flow cytometry, Hypericum linarioides bosse, Lc-Ms, Ms, Molecular docking
Kaynak
Chemistryselect
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
7
Sayı
23
Künye
This work was financially supported by grants from Erzincan University Scientific Research Projects Coordination Commission (EU-BAP) (Project No: 2020-736). The authors thank Esin Ak Yalcin and the research group for technical assistance. The authors also thank Prof. Dr. Ali Kandemir for plant identification.
