Separation, optimization, and quantification of cytokinins by a recently developed amide-embedded stationary phase
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Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, some plant growth regulators known as cytokinins [kinetin (K), zeatin (Z), thidiazuron (TDZ), benzylaminopurine (BAP), and dimethylallylaminopurine (AAP)] were separated by HPLC using an amide-embedded mixed-mode stationary phase which was synthesized by Aral et al. in recent years. The effect of mobile phase content, mobile phase pH, buffer concentration, and temperature on separation process was studied. In addition, a quantitative determination of cytokinins from Salvia limbata extract was studied, and some validation parameters such as limit of detection (LOD), limit of quantification (LOQ), and relative standard deviation (RSD) were calculated as a range of 0.03-0.1, 0.1-0.26mg/L, and 0.03-0.08, respectively. [GRAPHICS] .
Açıklama
Anahtar Kelimeler
Amide-Embedded Stationary Phase, Cytokinins, Mixed-Mode Stationary Phase, Plant Growth Regulators, Salvia Limbata
Kaynak
Journal of Liquid Chromatography & Related Technologies
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
40
Sayı
11
