Investigation of precore/core mutation of Hepatitis B virus with line probe immune assay method
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Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SCI Printers & Publication INC
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
OBJECTIVE: The expression of hepatitis B e antigen (HBeAg) is reduced or totally inhibited as a result of mutations in the precore/core region of the hepatitis B virus (HBV) genome, but its clinical significance has not been yet fully understood. In this study, we aimed to investigate precore/core mutation in serum samples of patients followed with a chronic hepatitis B infection diagnosis and whose HBeAg was found to be negative on ELISA test.
STUDY DESIGN: Among the chronic hepatitis B pa-tients followed in our hospital, serum samples of 111 patients with HBeAg negative were included in the study. In the serums of the patients whose HBV DNA were found positive with real-time PCR assay were investigated ''precore, core" mutations with the INNO-LiPA method.
RESULTS: HBV DNA was detected as positive in 93 serum samples. In 42 of those 93 (45.2%) serums, pre- core/core mutations were detected. Isolated precore mu-tation in 12 cases (12.9%), isolated core mutation in 9 cases (9.7%), and both precore and core mutations in 21 cases (22.6%) were found.
CONCLUSION: Mutant strains may play an important role in becoming chronic, hepato-carcinogenesis, and in the development of fulminant hepatitis or asymptomatic course. Until valid results are obtained revealing the otherwise, it must be kept in mind that there might be a risk factor of the nucleotide changes in the core re-gion associated with the activation of hepatitis B.
Açıklama
WOS:000765832800001
Anahtar Kelimeler
Basal core promoter, Genotyping techniques, HBeAg, Hepatitis B chronic, Hepatitis B core antigens, Hepatitis B e antigens, Hepatitis B virus, INNO-LiPA, Liver diseases, Precore/core mutation, Promoter regions, Genetic
Kaynak
Analytical and Quantitave Cytopathology and Histopathology
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
43
Sayı
5
Künye
Özbek, E., Temiz, H., Özcan, N. ve Özekinci, T. (2021). Investigation of precore/core mutation of Hepatitis B virus with line probe immune assay method. Analytical and Quantitave Cytopathology and Histopathology, 43(5), 289-295.
