Protective Ability of Ethanol Extracts of Hypericum scabroides Robson & Poulter and Hypericum triquetrifolium Turra against Protein Oxidation and DNA Damage
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Tarih
2009
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Korean Society Food Science & Technology-Kosfost
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The present study was conducted to determine the protective ability of the ethanol extracts of Hypericum scabroides Robson & Poulter (HS) and Hypericum triquetrifolium Turra (HT) against the protein oxidation and DNA damage induced by Fenton system. The ability of HS and HT to prevent oxidative damage to bovine serum albumin (BSA) induced by Fe3+/H2O2 and ascorbic acid was investigated. The ethanol extracts of HS and HT at different concentrations (50-1,000 mu g/mL) efficiently prevented protein oxidation induced by hydroxy radical as assayed by protein oxidation markers including protein carbonyl fort-nation (PCO) and polyacrylamide gel electrophoresis. The effect of ethanol extracts of HS and HT on DNA cleavage induced by UV-photholysis of H2O2 using pBluescript M13+ plasmid DNA were investigated. These extracts significantly inhibited DNA damage induced by reactive oxygen species (ROS). Therefore, HS and HT extracts may be useful in the food industry as effective synthetic antioxidants.
Açıklama
Anahtar Kelimeler
Protein Oxidation, Dna Damage, Fenton Chemistry, Hypericum Scabroides, Hypericum Triquetrifolium
Kaynak
Food Science and Biotechnology
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
18
Sayı
1
