Preparation and Use of Poly(hydroxyethyl methacrylate) Cryogels Containing L-Histidine for ?-Casein Adsorption

[ X ]

Tarih

2013

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Wiley-Blackwell

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

The objective of this study was to determine -casein adsorption by using supermacroporous poly(2-hydroxyethyl methacrylate-N-methacryloyl-(l)-histidine methyl ester) [p(HEMA-MAH)] cryogel. -Casein adsorption properties of p(HEMA-MAH) cryogel were studied for the application of -casein purification. The cryogel was produced by free radical polymerization initiated by N,N,N',N'-tetramethylene diamine and ammonium persulfate pairs in an ice bath. P(HEMA-MAH) cryogel was characterized by swelling tests, Fourier transform infrared spectroscopy, and scanning electron microscopy. The effects of the flow rate, pH, temperature, initial -casein concentration, and ionic strength on the adsorption efficiency of cryogel were studied. The equilibrium swelling degree of the p(HEMA-MAH) cryogel was 6.73g H2O/g cryogel. -Casein adsorption capacity of p(HEMA-MAH) cryogel from aqueous solution was estimated as 31.17mg/g cryogel. It was also observed that -casein could be repeatedly adsorbed and desorbed with p(HEMA-MAH) cryogel without significant loss in the adsorption capacity.

Açıklama

Anahtar Kelimeler

Adsorption, -Casein, Chromatography, Cryogel, P(Hema-Mah)

Kaynak

Journal of Food Science

WoS Q Değeri

Q2

Scopus Q Değeri

Q1

Cilt

78

Sayı

2

Künye