Preparation and Use of Poly(hydroxyethyl methacrylate) Cryogels Containing L-Histidine for ?-Casein Adsorption
[ X ]
Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-Blackwell
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The objective of this study was to determine -casein adsorption by using supermacroporous poly(2-hydroxyethyl methacrylate-N-methacryloyl-(l)-histidine methyl ester) [p(HEMA-MAH)] cryogel. -Casein adsorption properties of p(HEMA-MAH) cryogel were studied for the application of -casein purification. The cryogel was produced by free radical polymerization initiated by N,N,N',N'-tetramethylene diamine and ammonium persulfate pairs in an ice bath. P(HEMA-MAH) cryogel was characterized by swelling tests, Fourier transform infrared spectroscopy, and scanning electron microscopy. The effects of the flow rate, pH, temperature, initial -casein concentration, and ionic strength on the adsorption efficiency of cryogel were studied. The equilibrium swelling degree of the p(HEMA-MAH) cryogel was 6.73g H2O/g cryogel. -Casein adsorption capacity of p(HEMA-MAH) cryogel from aqueous solution was estimated as 31.17mg/g cryogel. It was also observed that -casein could be repeatedly adsorbed and desorbed with p(HEMA-MAH) cryogel without significant loss in the adsorption capacity.
Açıklama
Anahtar Kelimeler
Adsorption, -Casein, Chromatography, Cryogel, P(Hema-Mah)
Kaynak
Journal of Food Science
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
78
Sayı
2
