A Comprehensive study on a medicinal and edible plant scorzonera incisa DC., discussing the natural limits of phytochemical profiling
Yükleniyor...
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Leaves of Scorzonera incisa DC. (Asteraceae) are eaten raw against diabetes and headache. Evaluation of medicinal, edible plants using profiling methods has become a common phenomenon. Petroleum ether (PE), ethyl acetate (EA), and nbutanol (BU) fractions were prepared from ethanol extract of the aerial parts of the plant. Isolation by HPLC and phenolic profiling by validated LC-MS/MS method with 53 compounds were conducted on the EA fraction. GC-MS method was used to profile the PE fraction. In-vitro inhibitory activities of all fractions against α-amylase, α-glucosidase, AChE, BChE, tyrosinase and urease enzymes were measured. Isoorientin, vitexin, cynaroside and chlorogenic acid were isolated and characterized from the EA fraction as major compounds. LC-MS/MS analysis quantified 36 compounds in the EA fraction. 20 compounds were tentatively determined in the PE fraction. Compounds with the same molecular and fragment ions, a low percentage of identified compounds, and very close polarities were revealed to be some limits of the nature for LC-MS/MS profiling. Particularly, the presence and amount of substances other than the standard compounds used in the profiling methods and possible misdetections should not be neglected in evaluating medicinal plants and establishing a correlation between compounds and bioactivity.
Açıklama
Anahtar Kelimeler
Scorzonera incisa, Phytochemical Profiling
Kaynak
ChemistrySelect
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
8
Sayı
34
Künye
Şahin, H., Demir, S., Boğa, M., Sarı, A., Makbul, S. ve Coşkunçelebi, K. (2023). A Comprehensive study on a medicinal and edible plant scorzonera incisa DC., discussing the natural limits of phytochemical profiling. ChemistrySelect, 8(34), e202302173.
