Melatoninin sıçanlarda oluşturulan intestinal iskemi-reperfüzyon hasarlarında izole ileum düz kasfonksiyonel yanıtları üzerine etkisi
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Tarih
2014
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Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Bu çalışmanın amacı iyi bir antioksidan olarak bilinen melatoninin sıçanlarda oluşturulan intestinal iskemi-reperfüzyon hasarında izole ileum segmentlerinde düz kas fonksiyonel yanıtlarına etkisini araştırmaktır. Materyal ve Metod: Toplam 48 Sprague-Dawley rat 6 eşit gruba ayrıldı: Grup 1 kontrol, Grup 2 sham-opere, Grup 3 iskemi, Grup 4 iskemi-reperfüzyon, Grup 5 iskemi + melatonin, Grup 6 iskemi-reperfüzyon + melatonin. Melatonin intraperitoneal olarak 10 mg/kg dozunda verildi. Barsaklarda 30 dakika iskemi, 90 dakika reperfüzyon sağlandı. Ratlar ileum segmenti alındıktan sonra sakrifiye edildiler. Alman ileum segmenti izole organ banyosunda asıldı ve asetilkoline karşı kasılma, adrenaline karşı gevşeme yanıtlan kaydedildi. Ayrıca ileum segmentleri histopatolojik olarak incelendi. Bulgular: Asetilkolin kasılma yanıtları açısından, Grup 2, grup 3 ve grup 4 ile karşılaştırıldığı zaman istatistiki olarak anlamlı bulundu (p<0.05, p< 0.01). Grup 3, Grup 5 ile karşılaştırıldığı zaman anlamlı bulundu (p< 0.01). Grup 4 ile Grup 6 karşılaştırıldığı zaman istatistiki olarak anlamlı bulundu (pO.01). Adrenalin gevşeme yanıtları açısından, Grup 2, Grup 3 ve Grup 4 ile karşılaştırıldığı zaman istatistiki olarak anlamlı bulundu (sırasıyla p<0.05, p< 0.01). Grup 3, Grup 5 ile anlamlı sonuç verdi (p<0.05, pO.01). Grup 4 ile Grup 6 run karşılaştırılması istatistiki olarak anlamlıydı (p<0.05). Histopatolojik sonuçlar kasılma ve gevşeme yamtlarıyla uyumluydu. Sonuçlar: Bu çalışmada iskemi-reperfüzyona maruz kalan ratlann barsak dokusunun kasılma ve gevşeme yanıtları, kontrol ve sham öpere grubu kasılma ve gevşeme yanıtlarına göre daha düşük bulundu. İskemi-reperfüzyondan önce melatoninle tedavi ileal segmentlerin düz kas yanıtlarında bir düzelme sağladı.
The aim of this study was to determine the effect of melatonin, a hormone that is known as an antioxidant, on functional responses of isolated ileal smooth muscle in rat intestinal ischemia-reperfusion Materials and Methods: A total of 48 Sprague-Dawley rats were divided equally into 6 groups: Group 1 was control, Group 2 was sham-operated, Group 3 was ischemia, Group 4 was ischemia-reperfusion, Group 5 was ischemia plus melatonin, and Group 6 was ischemia- reperfusion plus melatonin. A dose of 10 mg/kg melatonin was administered intraperitoneally. 30 minutes before intestinal ischemia was followed by 90 minutes of reperfusion. Rats were sacrified after removal of ileal segments. Ileal segments were placed in isolated organ baths and contraction responses for acetylcholine and relaxation responses for adrenaline were recorded. Additionally, ileal segments were examined histopathologically. Results : By t he c ontraction r esponses of a cetylcholine, w hile G roup 2 w as c ompared t o Group 3 and Group 4, the differences between these groups were found significant (p<0.05, p< 0.01). Also the differences between Group 3 and Group 4, Group 5 were found significant (p<0.01). The comparison of Group 4 and Group 6 was found significant (pO.Ol). Comparison of relaxation adrenaline responses of Group 2 with Group 3 and Group 4 was found significant (respectively p<0.05, p< 0.01). The comparison of Group 3 and Group 5 was also found significant (p<0.05, pO.Ol). The comparison of Group 4 and Group 6 was found significant (p<0.05). The histopathologic results were consistent with responses of contraction and relaxation. Conclusions: The results of this study showed that the contraction and relaxation responses of rat ileal tissue exposed to ischemia-reperfusion were lower than those of control and sham- operated group. Treatment with melatonin before ischemia-reperfusion improved the responses of ileal tissues
The aim of this study was to determine the effect of melatonin, a hormone that is known as an antioxidant, on functional responses of isolated ileal smooth muscle in rat intestinal ischemia-reperfusion Materials and Methods: A total of 48 Sprague-Dawley rats were divided equally into 6 groups: Group 1 was control, Group 2 was sham-operated, Group 3 was ischemia, Group 4 was ischemia-reperfusion, Group 5 was ischemia plus melatonin, and Group 6 was ischemia- reperfusion plus melatonin. A dose of 10 mg/kg melatonin was administered intraperitoneally. 30 minutes before intestinal ischemia was followed by 90 minutes of reperfusion. Rats were sacrified after removal of ileal segments. Ileal segments were placed in isolated organ baths and contraction responses for acetylcholine and relaxation responses for adrenaline were recorded. Additionally, ileal segments were examined histopathologically. Results : By t he c ontraction r esponses of a cetylcholine, w hile G roup 2 w as c ompared t o Group 3 and Group 4, the differences between these groups were found significant (p<0.05, p< 0.01). Also the differences between Group 3 and Group 4, Group 5 were found significant (p<0.01). The comparison of Group 4 and Group 6 was found significant (pO.Ol). Comparison of relaxation adrenaline responses of Group 2 with Group 3 and Group 4 was found significant (respectively p<0.05, p< 0.01). The comparison of Group 3 and Group 5 was also found significant (p<0.05, pO.Ol). The comparison of Group 4 and Group 6 was found significant (p<0.05). The histopathologic results were consistent with responses of contraction and relaxation. Conclusions: The results of this study showed that the contraction and relaxation responses of rat ileal tissue exposed to ischemia-reperfusion were lower than those of control and sham- operated group. Treatment with melatonin before ischemia-reperfusion improved the responses of ileal tissues
Açıklama
Anahtar Kelimeler
Eczacılık , Farmakoloji ,hareket sistemi, iskemi-reperfüzyon, melatonin,izole ileum, rat, ischemia-reperfusion, melatonin, isolated ileum, rat.
